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• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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Hypothesis Examples

A hypothesis is a prediction of the outcome of a test. It forms the basis for designing an experiment in the scientific method . A good hypothesis is testable, meaning it makes a prediction you can check with observation or experimentation. Here are different hypothesis examples.

Null Hypothesis Examples

The null hypothesis (H 0 ) is also known as the zero-difference or no-difference hypothesis. It predicts that changing one variable ( independent variable ) will have no effect on the variable being measured ( dependent variable ). Here are null hypothesis examples:

• Plant growth is unaffected by temperature.
• If you increase temperature, then solubility of salt will increase.
• Incidence of skin cancer is unrelated to ultraviolet light exposure.
• All brands of light bulb last equally long.
• Cats have no preference for the color of cat food.
• All daisies have the same number of petals.

Sometimes the null hypothesis shows there is a suspected correlation between two variables. For example, if you think plant growth is affected by temperature, you state the null hypothesis: “Plant growth is not affected by temperature.” Why do you do this, rather than say “If you change temperature, plant growth will be affected”? The answer is because it’s easier applying a statistical test that shows, with a high level of confidence, a null hypothesis is correct or incorrect.

Research Hypothesis Examples

A research hypothesis (H 1 ) is a type of hypothesis used to design an experiment. This type of hypothesis is often written as an if-then statement because it’s easy identifying the independent and dependent variables and seeing how one affects the other. If-then statements explore cause and effect. In other cases, the hypothesis shows a correlation between two variables. Here are some research hypothesis examples:

• If you leave the lights on, then it takes longer for people to fall asleep.
• If you refrigerate apples, they last longer before going bad.
• If you keep the curtains closed, then you need less electricity to heat or cool the house (the electric bill is lower).
• If you leave a bucket of water uncovered, then it evaporates more quickly.
• Goldfish lose their color if they are not exposed to light.
• Workers who take vacations are more productive than those who never take time off.

Is It Okay to Disprove a Hypothesis?

Yes! You may even choose to write your hypothesis in such a way that it can be disproved because it’s easier to prove a statement is wrong than to prove it is right. In other cases, if your prediction is incorrect, that doesn’t mean the science is bad. Revising a hypothesis is common. It demonstrates you learned something you did not know before you conducted the experiment.

Test yourself with a Scientific Method Quiz .

• Mellenbergh, G.J. (2008). Chapter 8: Research designs: Testing of research hypotheses. In H.J. Adèr & G.J. Mellenbergh (eds.), Advising on Research Methods: A Consultant’s Companion . Huizen, The Netherlands: Johannes van Kessel Publishing.
• Popper, Karl R. (1959). The Logic of Scientific Discovery . Hutchinson & Co. ISBN 3-1614-8410-X.
• Schick, Theodore; Vaughn, Lewis (2002). How to think about weird things: critical thinking for a New Age . Boston: McGraw-Hill Higher Education. ISBN 0-7674-2048-9.
• Tobi, Hilde; Kampen, Jarl K. (2018). “Research design: the methodology for interdisciplinary research framework”. Quality & Quantity . 52 (3): 1209–1225. doi: 10.1007/s11135-017-0513-8

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scientific hypothesis

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• National Center for Biotechnology Information - PubMed Central - On the scope of scientific hypotheses
• LiveScience - What is a scientific hypothesis?
• The Royal Society - Open Science - On the scope of scientific hypotheses

scientific hypothesis , an idea that proposes a tentative explanation about a phenomenon or a narrow set of phenomena observed in the natural world. The two primary features of a scientific hypothesis are falsifiability and testability, which are reflected in an “If…then” statement summarizing the idea and in the ability to be supported or refuted through observation and experimentation. The notion of the scientific hypothesis as both falsifiable and testable was advanced in the mid-20th century by Austrian-born British philosopher Karl Popper .

The formulation and testing of a hypothesis is part of the scientific method , the approach scientists use when attempting to understand and test ideas about natural phenomena. The generation of a hypothesis frequently is described as a creative process and is based on existing scientific knowledge, intuition , or experience. Therefore, although scientific hypotheses commonly are described as educated guesses, they actually are more informed than a guess. In addition, scientists generally strive to develop simple hypotheses, since these are easier to test relative to hypotheses that involve many different variables and potential outcomes. Such complex hypotheses may be developed as scientific models ( see scientific modeling ).

Depending on the results of scientific evaluation, a hypothesis typically is either rejected as false or accepted as true. However, because a hypothesis inherently is falsifiable, even hypotheses supported by scientific evidence and accepted as true are susceptible to rejection later, when new evidence has become available. In some instances, rather than rejecting a hypothesis because it has been falsified by new evidence, scientists simply adapt the existing idea to accommodate the new information. In this sense a hypothesis is never incorrect but only incomplete.

The investigation of scientific hypotheses is an important component in the development of scientific theory . Hence, hypotheses differ fundamentally from theories; whereas the former is a specific tentative explanation and serves as the main tool by which scientists gather data, the latter is a broad general explanation that incorporates data from many different scientific investigations undertaken to explore hypotheses.

Countless hypotheses have been developed and tested throughout the history of science . Several examples include the idea that living organisms develop from nonliving matter, which formed the basis of spontaneous generation , a hypothesis that ultimately was disproved (first in 1668, with the experiments of Italian physician Francesco Redi , and later in 1859, with the experiments of French chemist and microbiologist Louis Pasteur ); the concept proposed in the late 19th century that microorganisms cause certain diseases (now known as germ theory ); and the notion that oceanic crust forms along submarine mountain zones and spreads laterally away from them ( seafloor spreading hypothesis ).

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• Knowledge Base
• Methodology
• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

Prevent plagiarism, run a free check.

Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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McCombes, S. (2022, May 06). How to Write a Strong Hypothesis | Guide & Examples. Scribbr. Retrieved 10 July 2024, from https://www.scribbr.co.uk/research-methods/hypothesis-writing/

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What is and How to Write a Good Hypothesis in Research?

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Table of Contents

One of the most important aspects of conducting research is constructing a strong hypothesis. But what makes a hypothesis in research effective? In this article, we’ll look at the difference between a hypothesis and a research question, as well as the elements of a good hypothesis in research. We’ll also include some examples of effective hypotheses, and what pitfalls to avoid.

What is a Hypothesis in Research?

Simply put, a hypothesis is a research question that also includes the predicted or expected result of the research. Without a hypothesis, there can be no basis for a scientific or research experiment. As such, it is critical that you carefully construct your hypothesis by being deliberate and thorough, even before you set pen to paper. Unless your hypothesis is clearly and carefully constructed, any flaw can have an adverse, and even grave, effect on the quality of your experiment and its subsequent results.

Research Question vs Hypothesis

It’s easy to confuse research questions with hypotheses, and vice versa. While they’re both critical to the Scientific Method, they have very specific differences. Primarily, a research question, just like a hypothesis, is focused and concise. But a hypothesis includes a prediction based on the proposed research, and is designed to forecast the relationship of and between two (or more) variables. Research questions are open-ended, and invite debate and discussion, while hypotheses are closed, e.g. “The relationship between A and B will be C.”

A hypothesis is generally used if your research topic is fairly well established, and you are relatively certain about the relationship between the variables that will be presented in your research. Since a hypothesis is ideally suited for experimental studies, it will, by its very existence, affect the design of your experiment. The research question is typically used for new topics that have not yet been researched extensively. Here, the relationship between different variables is less known. There is no prediction made, but there may be variables explored. The research question can be casual in nature, simply trying to understand if a relationship even exists, descriptive or comparative.

How to Write Hypothesis in Research

Writing an effective hypothesis starts before you even begin to type. Like any task, preparation is key, so you start first by conducting research yourself, and reading all you can about the topic that you plan to research. From there, you’ll gain the knowledge you need to understand where your focus within the topic will lie.

Remember that a hypothesis is a prediction of the relationship that exists between two or more variables. Your job is to write a hypothesis, and design the research, to “prove” whether or not your prediction is correct. A common pitfall is to use judgments that are subjective and inappropriate for the construction of a hypothesis. It’s important to keep the focus and language of your hypothesis objective.

An effective hypothesis in research is clearly and concisely written, and any terms or definitions clarified and defined. Specific language must also be used to avoid any generalities or assumptions.

Use the following points as a checklist to evaluate the effectiveness of your research hypothesis:

• Predicts the relationship and outcome
• Simple and concise – avoid wordiness
• Clear with no ambiguity or assumptions about the readers’ knowledge
• Observable and testable results
• Relevant and specific to the research question or problem

Research Hypothesis Example

Perhaps the best way to evaluate whether or not your hypothesis is effective is to compare it to those of your colleagues in the field. There is no need to reinvent the wheel when it comes to writing a powerful research hypothesis. As you’re reading and preparing your hypothesis, you’ll also read other hypotheses. These can help guide you on what works, and what doesn’t, when it comes to writing a strong research hypothesis.

Here are a few generic examples to get you started.

Eating an apple each day, after the age of 60, will result in a reduction of frequency of physician visits.

Budget airlines are more likely to receive more customer complaints. A budget airline is defined as an airline that offers lower fares and fewer amenities than a traditional full-service airline. (Note that the term “budget airline” is included in the hypothesis.

Workplaces that offer flexible working hours report higher levels of employee job satisfaction than workplaces with fixed hours.

Each of the above examples are specific, observable and measurable, and the statement of prediction can be verified or shown to be false by utilizing standard experimental practices. It should be noted, however, that often your hypothesis will change as your research progresses.

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Elsevier’s Language Editing Plus service can help ensure that your research hypothesis is well-designed, and articulates your research and conclusions. Our most comprehensive editing package, you can count on a thorough language review by native-English speakers who are PhDs or PhD candidates. We’ll check for effective logic and flow of your manuscript, as well as document formatting for your chosen journal, reference checks, and much more.

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What makes a good hypothesis?

Formulating a good hypothesis is the backbone of the scientific method.

A hypothesis is a precise and testable statement of what a researcher predicts will be the outcome of a study. This usually involves proposing a relationship between two or more variables.

Verifying a hypothesis, also sometimes referred to as a working statement , requires using the scientific method , usually by designing an experiment.

For instance, one common adage is ‘an apple a day keeps the doctor away’. If we use this aphorism as our hypothesis then we can make a prediction that consuming at least one apple per day should result in fewer visits to the doctor than the general population that eats apples sparingly or never.

In 2015 , researchers at Dartmouth College, the University of Michigan School of Nursing, and the Veteran Affairs Medical Center in White River actually investigated this hypothesis. They combed national nutrition data collected from nearly 8,400 men and women — 753 of whom ate an apple a day. The study found that “evidence does not support that an apple a day keeps the doctor away; however, the small fraction of US adults who eat an apple a day do appear to use fewer prescription medications.”

So perhaps there’s a glimmer of truth to this hypothesis, but not necessarily because apples are some miracle foods. It could be that people who eat apples every day also consume other fresh produce and less processed foods than the general population, a diet that helps to prevent obesity, a huge risk factor for a myriad of illnesses such as hypertension and diabetes that require prescription medication. This is why hypotheses need to be defined as precisely and as narrowly as possible in order to isolate confounding effects.

Types of hypothesis

The ‘apple a day’ study is an example of an alternative hypothesis , which states that there is a relationship between two variables being studied, the daily apple consumption and visits to the GP. One variable, called the independent variable , has an effect on the other, known as the dependent variable . The independent variable is what you change and the dependent variable is what you measure. For example, if I am measuring how a plant grows with different fertilizers, the fertilizers are what I can change freely (independent) while the plant’s growth would be dependent on what it is given. In order for an alternative hypothesis to be validated, the results have to have statistical significance in order to rule out chance.

Examples of alternative hypotheses:

• Dogs wag their tails when they’re happy.
• The accumulation of greenhouse gases in the atmosphere raises global average temperature.
• Wearing a seatbelt reduces traffic-related fatalities.
• Students who attend class earn higher scores than students who skip class.
• People exposed to higher levels of UV light have a higher incidence of skin cancer than the general population.

Another common type of hypothesis used in science is the null hypothesis , which states that there is no relationship between two variables. This means that controlling one variable has no effect on the other. Any results are due to chance and thus pursuing a cause-effect relationship between the two variables is futile.

The null hypothesis is the polar opposite of the alternative hypothesis since they contain opposing viewpoints. In fact, the latter is called this way because it is an alternative to the null hypothesis. An apple a day doesn’t keep the doctor away, you could propose if you were designing a null hypothesis experiment.

Examples of null hypotheses:

• Taking an aspirin a day doesn’t reduce the risk of a heart attack.
• Playing classical music doesn’t help plants grow more biomass.
• Vaccines don’t cause autism.
• Hyperactivity is unrelated to sugar consumption.

The acceptance of the alternative hypothesis, often denoted by H 1 , depends on the rejection of the null hypothesis (H 0 ). A null hypothesis can never be proven, it can only be rejected. To test a null hypothesis and determine whether the observed data is not due to change or the manipulation of data, scientists employ a significance test.

Rejecting the null hypothesis does not necessarily imply that a study did not produce the required results. Instead, it sets the stage for further experimentation to see if a relationship between the two variables truly exists.

For instance, say a scientist proposes a null hypothesis stating that “the rate of plant growth is not affected by sunlight.” One way to investigate this conjecture would be to monitor a random sample of plants grown with or without sunlight. You then measure the average mass of each group of plants and if there’s a statistically significant difference in the observed change, then the null hypothesis is rejected. Consequently, the alternate hypothesis that “plant growth is affected by sunlight” is accepted, then scientists can perform further research into the effects of different wavelengths of light or intensities of light on plant growth.

At this point, you might be wondering why we need the null hypothesis. Why not propose and test an alternate hypothesis and see if it is true? One explanation is that science cannot provide absolute proofs, but rather approximations. The scientific method cannot explicitly “prove” propositions. We can never prove an alternative hypothesis with 100% confidence. What we can do instead is reject the null hypothesis, supporting the alternative hypothesis.

It just so happens that it is easier to disprove a hypothesis than to positively prove one. But the supposition that the null hypothesis is incorrect allows for a stable foundation on which scientists can build. You can view it this way: the results from testing the null hypothesis lay the groundwork for the alternate hypothesis, which explores multiple ideas that may or may not be correct.

The alternative and null hypotheses are the two main types you’ll encounter in studies. But the alternative hypothesis can be further broken down into two categories: directional and nondirectional alternative hypotheses.

The directional alternative hypothesis predicts that the independent variable will have an effect on the dependent variable and the direction in which the change will take place. The nondirectional alternative hypothesis predicts the independent variable will have an effect but its direction is not specific, without stating the magnitude of the difference.

For instance, a non-directional hypothesis could be “there will be a difference in how many words children and adults can recall,” while the directional hypothesis could predict that “adults will recall more words than children.”

Hypotheses can be simple or complex. A simple hypothesis predicts a relationship between a single dependent variable and a single independent variable while a complex one predicts a relationship between two or more independent and dependent variables. An example of a complex hypothesis could be “Do age and weight affect the chances of getting diabetes and heart diseases?” There are two independent and two dependent variables in this statement whose relationship we seek to verify.

How to write a good hypothesis

The way you formulate a hypothesis can make or break your research because the validity of an experiment and its results rely heavily on a robust testable hypothesis. A good research hypothesis typically involves more effort than a simple guess or assumption.

Generally, a good hypothesis:

• is testable, meaning it must be possible to show that a hypothesis is true or false, and the results of this investigation have to be replicable;
• includes both an independent and dependent variable.
• allows for the manipulation of the variables ethically.
• has clear and focused language. Don’t be vague.
• is related to other published research.
• is written, either explicitly or not, as an “if-then” statement because we can then make a prediction of the outcome of an experiment.

An example of a testable good hypothesis is a conjecture such as “Students recall more information during the afternoon than during the morning.” The independent variable is the time of the lecture and the dependent variable is the recall of the information presented in the lecture, which can be verified with standardized tests.

A bad hypothesis could be something like “Goldfish make better pets than cats.” Right off the bat, you can see a couple of problems with this statement. What constitutes a good pet? Is a good pet fluffy and interactive or one that is low maintenance? Can I predict whether a cat or goldfish will make for a good pet? This is more a matter of opinion that doesn’t provide any meaningful results.

Often, the best hypotheses start from observation. For instance, everybody has witnessed that objects that are thrown into the air will fall toward the ground. Sir Isaac Newton formulated a hypothesis in the 17th-century that explains this observation, stating that ‘objects with mass attract each other through a gravitational field.’

But despite Newton’s hypothesis being very well written, in the sense that it is testable, simple, clear, and universal, we now know it was wrong. In the 20th-century, Albert Einstein showed that a hypothesis that more precisely explains the observed phenomenon is that ‘objects with mass cause space to bend.’ The lesson here is that all hypotheses are temporary and partial, they’re never permanent and irrefutable. This is also a good example of why the null hypothesis is so paramount.

Hypothesis formulation and testing through statistical methods are integral parts of the scientific method, the systematic approach to assessing whether a statement is true or false. All the best stories in science start with a good hypothesis. 

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• v.34(45); 2019 Nov 25

Scientific Hypotheses: Writing, Promoting, and Predicting Implications

Armen yuri gasparyan.

1 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, West Midlands, UK.

Lilit Ayvazyan

2 Department of Medical Chemistry, Yerevan State Medical University, Yerevan, Armenia.

Ulzhan Mukanova

3 Department of Surgical Disciplines, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

George D. Kitas

5 Arthritis Research UK Epidemiology Unit, University of Manchester, Manchester, UK.

Scientific hypotheses are essential for progress in rapidly developing academic disciplines. Proposing new ideas and hypotheses require thorough analyses of evidence-based data and predictions of the implications. One of the main concerns relates to the ethical implications of the generated hypotheses. The authors may need to outline potential benefits and limitations of their suggestions and target widely visible publication outlets to ignite discussion by experts and start testing the hypotheses. Not many publication outlets are currently welcoming hypotheses and unconventional ideas that may open gates to criticism and conservative remarks. A few scholarly journals guide the authors on how to structure hypotheses. Reflecting on general and specific issues around the subject matter is often recommended for drafting a well-structured hypothesis article. An analysis of influential hypotheses, presented in this article, particularly Strachan's hygiene hypothesis with global implications in the field of immunology and allergy, points to the need for properly interpreting and testing new suggestions. Envisaging the ethical implications of the hypotheses should be considered both by authors and journal editors during the writing and publishing process.

INTRODUCTION

We live in times of digitization that radically changes scientific research, reporting, and publishing strategies. Researchers all over the world are overwhelmed with processing large volumes of information and searching through numerous online platforms, all of which make the whole process of scholarly analysis and synthesis complex and sophisticated.

Current research activities are diversifying to combine scientific observations with analysis of facts recorded by scholars from various professional backgrounds. 1 Citation analyses and networking on social media are also becoming essential for shaping research and publishing strategies globally. 2 Learning specifics of increasingly interdisciplinary research studies and acquiring information facilitation skills aid researchers in formulating innovative ideas and predicting developments in interrelated scientific fields.

Arguably, researchers are currently offered more opportunities than in the past for generating new ideas by performing their routine laboratory activities, observing individual cases and unusual developments, and critically analyzing published scientific facts. What they need at the start of their research is to formulate a scientific hypothesis that revisits conventional theories, real-world processes, and related evidence to propose new studies and test ideas in an ethical way. 3 Such a hypothesis can be of most benefit if published in an ethical journal with wide visibility and exposure to relevant online databases and promotion platforms.

Although hypotheses are crucially important for the scientific progress, only few highly skilled researchers formulate and eventually publish their innovative ideas per se . Understandably, in an increasingly competitive research environment, most authors would prefer to prioritize their ideas by discussing and conducting tests in their own laboratories or clinical departments, and publishing research reports afterwards. However, there are instances when simple observations and research studies in a single center are not capable of explaining and testing new groundbreaking ideas. Formulating hypothesis articles first and calling for multicenter and interdisciplinary research can be a solution in such instances, potentially launching influential scientific directions, if not academic disciplines.

The aim of this article is to overview the importance and implications of infrequently published scientific hypotheses that may open new avenues of thinking and research.

Despite the seemingly established views on innovative ideas and hypotheses as essential research tools, no structured definition exists to tag the term and systematically track related articles. In 1973, the Medical Subject Heading (MeSH) of the U.S. National Library of Medicine introduced “Research Design” as a structured keyword that referred to the importance of collecting data and properly testing hypotheses, and indirectly linked the term to ethics, methods and standards, among many other subheadings.

One of the experts in the field defines “hypothesis” as a well-argued analysis of available evidence to provide a realistic (scientific) explanation of existing facts, fill gaps in public understanding of sophisticated processes, and propose a new theory or a test. 4 A hypothesis can be proven wrong partially or entirely. However, even such an erroneous hypothesis may influence progress in science by initiating professional debates that help generate more realistic ideas. The main ethical requirement for hypothesis authors is to be honest about the limitations of their suggestions. 5

EXAMPLES OF INFLUENTIAL SCIENTIFIC HYPOTHESES

Daily routine in a research laboratory may lead to groundbreaking discoveries provided the daily accounts are comprehensively analyzed and reproduced by peers. The discovery of penicillin by Sir Alexander Fleming (1928) can be viewed as a prime example of such discoveries that introduced therapies to treat staphylococcal and streptococcal infections and modulate blood coagulation. 6 , 7 Penicillin got worldwide recognition due to the inventor's seminal works published by highly prestigious and widely visible British journals, effective ‘real-world’ antibiotic therapy of pneumonia and wounds during World War II, and euphoric media coverage. 8 In 1945, Fleming, Florey and Chain got a much deserved Nobel Prize in Physiology or Medicine for the discovery that led to the mass production of the wonder drug in the U.S. and ‘real-world practice’ that tested the use of penicillin. What remained globally unnoticed is that Zinaida Yermolyeva, the outstanding Soviet microbiologist, created the Soviet penicillin, which turned out to be more effective than the Anglo-American penicillin and entered mass production in 1943; that year marked the turning of the tide of the Great Patriotic War. 9 One of the reasons of the widely unnoticed discovery of Zinaida Yermolyeva is that her works were published exclusively by local Russian (Soviet) journals.

The past decades have been marked by an unprecedented growth of multicenter and global research studies involving hundreds and thousands of human subjects. This trend is shaped by an increasing number of reports on clinical trials and large cohort studies that create a strong evidence base for practice recommendations. Mega-studies may help generate and test large-scale hypotheses aiming to solve health issues globally. Properly designed epidemiological studies, for example, may introduce clarity to the hygiene hypothesis that was originally proposed by David Strachan in 1989. 10 David Strachan studied the epidemiology of hay fever in a cohort of 17,414 British children and concluded that declining family size and improved personal hygiene had reduced the chances of cross infections in families, resulting in epidemics of atopic disease in post-industrial Britain. Over the past four decades, several related hypotheses have been proposed to expand the potential role of symbiotic microorganisms and parasites in the development of human physiological immune responses early in life and protection from allergic and autoimmune diseases later on. 11 , 12 Given the popularity and the scientific importance of the hygiene hypothesis, it was introduced as a MeSH term in 2012. 13

Hypotheses can be proposed based on an analysis of recorded historic events that resulted in mass migrations and spreading of certain genetic diseases. As a prime example, familial Mediterranean fever (FMF), the prototype periodic fever syndrome, is believed to spread from Mesopotamia to the Mediterranean region and all over Europe due to migrations and religious prosecutions millennia ago. 14 Genetic mutations spearing mild clinical forms of FMF are hypothesized to emerge and persist in the Mediterranean region as protective factors against more serious infectious diseases, particularly tuberculosis, historically common in that part of the world. 15 The speculations over the advantages of carrying the MEditerranean FeVer (MEFV) gene are further strengthened by recorded low mortality rates from tuberculosis among FMF patients of different nationalities living in Tunisia in the first half of the 20th century. 16

Diagnostic hypotheses shedding light on peculiarities of diseases throughout the history of mankind can be formulated using artefacts, particularly historic paintings. 17 Such paintings may reveal joint deformities and disfigurements due to rheumatic diseases in individual subjects. A series of paintings with similar signs of pathological conditions interpreted in a historic context may uncover mysteries of epidemics of certain diseases, which is the case with Ruben's paintings depicting signs of rheumatic hands and making some doctors to believe that rheumatoid arthritis was common in Europe in the 16th and 17th century. 18

WRITING SCIENTIFIC HYPOTHESES

There are author instructions of a few journals that specifically guide how to structure, format, and make submissions categorized as hypotheses attractive. One of the examples is presented by Med Hypotheses , the flagship journal in its field with more than four decades of publishing and influencing hypothesis authors globally. However, such guidance is not based on widely discussed, implemented, and approved reporting standards, which are becoming mandatory for all scholarly journals.

Generating new ideas and scientific hypotheses is a sophisticated task since not all researchers and authors are skilled to plan, conduct, and interpret various research studies. Some experience with formulating focused research questions and strong working hypotheses of original research studies is definitely helpful for advancing critical appraisal skills. However, aspiring authors of scientific hypotheses may need something different, which is more related to discerning scientific facts, pooling homogenous data from primary research works, and synthesizing new information in a systematic way by analyzing similar sets of articles. To some extent, this activity is reminiscent of writing narrative and systematic reviews. As in the case of reviews, scientific hypotheses need to be formulated on the basis of comprehensive search strategies to retrieve all available studies on the topics of interest and then synthesize new information selectively referring to the most relevant items. One of the main differences between scientific hypothesis and review articles relates to the volume of supportive literature sources ( Table 1 ). In fact, hypothesis is usually formulated by referring to a few scientific facts or compelling evidence derived from a handful of literature sources. 19 By contrast, reviews require analyses of a large number of published documents retrieved from several well-organized and evidence-based databases in accordance with predefined search strategies. 20 , 21 , 22

The format of hypotheses, especially the implications part, may vary widely across disciplines. Clinicians may limit their suggestions to the clinical manifestations of diseases, outcomes, and management strategies. Basic and laboratory scientists analysing genetic, molecular, and biochemical mechanisms may need to view beyond the frames of their narrow fields and predict social and population-based implications of the proposed ideas. 23

Advanced writing skills are essential for presenting an interesting theoretical article which appeals to the global readership. Merely listing opposing facts and ideas, without proper interpretation and analysis, may distract the experienced readers. The essence of a great hypothesis is a story behind the scientific facts and evidence-based data.

ETHICAL IMPLICATIONS

The authors of hypotheses substantiate their arguments by referring to and discerning rational points from published articles that might be overlooked by others. Their arguments may contradict the established theories and practices, and pose global ethical issues, particularly when more or less efficient medical technologies and public health interventions are devalued. The ethical issues may arise primarily because of the careless references to articles with low priorities, inadequate and apparently unethical methodologies, and concealed reporting of negative results. 24 , 25

Misinterpretation and misunderstanding of the published ideas and scientific hypotheses may complicate the issue further. For example, Alexander Fleming, whose innovative ideas of penicillin use to kill susceptible bacteria saved millions of lives, warned of the consequences of uncontrolled prescription of the drug. The issue of antibiotic resistance had emerged within the first ten years of penicillin use on a global scale due to the overprescription that affected the efficacy of antibiotic therapies, with undesirable consequences for millions. 26

The misunderstanding of the hygiene hypothesis that primarily aimed to shed light on the role of the microbiome in allergic and autoimmune diseases resulted in decline of public confidence in hygiene with dire societal implications, forcing some experts to abandon the original idea. 27 , 28 Although that hypothesis is unrelated to the issue of vaccinations, the public misunderstanding has resulted in decline of vaccinations at a time of upsurge of old and new infections.

A number of ethical issues are posed by the denial of the viral (human immunodeficiency viruses; HIV) hypothesis of acquired Immune deficiency Syndrome (AIDS) by Peter Duesberg, who overviewed the links between illicit recreational drugs and antiretroviral therapies with AIDS and refuted the etiological role of HIV. 29 That controversial hypothesis was rejected by several journals, but was eventually published without external peer review at Med Hypotheses in 2010. The publication itself raised concerns of the unconventional editorial policy of the journal, causing major perturbations and more scrutinized publishing policies by journals processing hypotheses.

WHERE TO PUBLISH HYPOTHESES

Although scientific authors are currently well informed and equipped with search tools to draft evidence-based hypotheses, there are still limited quality publication outlets calling for related articles. The journal editors may be hesitant to publish articles that do not adhere to any research reporting guidelines and open gates for harsh criticism of unconventional and untested ideas. Occasionally, the editors opting for open-access publishing and upgrading their ethics regulations launch a section to selectively publish scientific hypotheses attractive to the experienced readers. 30 However, the absence of approved standards for this article type, particularly no mandate for outlining potential ethical implications, may lead to publication of potentially harmful ideas in an attractive format.

A suggestion of simultaneously publishing multiple or alternative hypotheses to balance the reader views and feedback is a potential solution for the mainstream scholarly journals. 31 However, that option alone is hardly applicable to emerging journals with unconventional quality checks and peer review, accumulating papers with multiple rejections by established journals.

A large group of experts view hypotheses with improbable and controversial ideas publishable after formal editorial (in-house) checks to preserve the authors' genuine ideas and avoid conservative amendments imposed by external peer reviewers. 32 That approach may be acceptable for established publishers with large teams of experienced editors. However, the same approach can lead to dire consequences if employed by nonselective start-up, open-access journals processing all types of articles and primarily accepting those with charged publication fees. 33 In fact, pseudoscientific ideas arguing Newton's and Einstein's seminal works or those denying climate change that are hardly testable have already found their niche in substandard electronic journals with soft or nonexistent peer review. 34

CITATIONS AND SOCIAL MEDIA ATTENTION

The available preliminary evidence points to the attractiveness of hypothesis articles for readers, particularly those from research-intensive countries who actively download related documents. 35 However, citations of such articles are disproportionately low. Only a small proportion of top-downloaded hypotheses (13%) in the highly prestigious Med Hypotheses receive on average 5 citations per article within a two-year window. 36

With the exception of a few historic papers, the vast majority of hypotheses attract relatively small number of citations in a long term. 36 Plausible explanations are that these articles often contain a single or only a few citable points and that suggested research studies to test hypotheses are rarely conducted and reported, limiting chances of citing and crediting authors of genuine research ideas.

A snapshot analysis of citation activity of hypothesis articles may reveal interest of the global scientific community towards their implications across various disciplines and countries. As a prime example, Strachan's hygiene hypothesis, published in 1989, 10 is still attracting numerous citations on Scopus, the largest bibliographic database. As of August 28, 2019, the number of the linked citations in the database is 3,201. Of the citing articles, 160 are cited at least 160 times ( h -index of this research topic = 160). The first three citations are recorded in 1992 and followed by a rapid annual increase in citation activity and a peak of 212 in 2015 ( Fig. 1 ). The top 5 sources of the citations are Clin Exp Allergy (n = 136), J Allergy Clin Immunol (n = 119), Allergy (n = 81), Pediatr Allergy Immunol (n = 69), and PLOS One (n = 44). The top 5 citing authors are leading experts in pediatrics and allergology Erika von Mutius (Munich, Germany, number of publications with the index citation = 30), Erika Isolauri (Turku, Finland, n = 27), Patrick G Holt (Subiaco, Australia, n = 25), David P. Strachan (London, UK, n = 23), and Bengt Björksten (Stockholm, Sweden, n = 22). The U.S. is the leading country in terms of citation activity with 809 related documents, followed by the UK (n = 494), Germany (n = 314), Australia (n = 211), and the Netherlands (n = 177). The largest proportion of citing documents are articles (n = 1,726, 54%), followed by reviews (n = 950, 29.7%), and book chapters (n = 213, 6.7%). The main subject areas of the citing items are medicine (n = 2,581, 51.7%), immunology and microbiology (n = 1,179, 23.6%), and biochemistry, genetics and molecular biology (n = 415, 8.3%).

Interestingly, a recent analysis of 111 publications related to Strachan's hygiene hypothesis, stating that the lack of exposure to infections in early life increases the risk of rhinitis, revealed a selection bias of 5,551 citations on Web of Science. 37 The articles supportive of the hypothesis were cited more than nonsupportive ones (odds ratio adjusted for study design, 2.2; 95% confidence interval, 1.6–3.1). A similar conclusion pointing to a citation bias distorting bibliometrics of hypotheses was reached by an earlier analysis of a citation network linked to the idea that β-amyloid, which is involved in the pathogenesis of Alzheimer disease, is produced by skeletal muscle of patients with inclusion body myositis. 38 The results of both studies are in line with the notion that ‘positive’ citations are more frequent in the field of biomedicine than ‘negative’ ones, and that citations to articles with proven hypotheses are too common. 39

Social media channels are playing an increasingly active role in the generation and evaluation of scientific hypotheses. In fact, publicly discussing research questions on platforms of news outlets, such as Reddit, may shape hypotheses on health-related issues of global importance, such as obesity. 40 Analyzing Twitter comments, researchers may reveal both potentially valuable ideas and unfounded claims that surround groundbreaking research ideas. 41 Social media activities, however, are unevenly distributed across different research topics, journals and countries, and these are not always objective professional reflections of the breakthroughs in science. 2 , 42

Scientific hypotheses are essential for progress in science and advances in healthcare. Innovative ideas should be based on a critical overview of related scientific facts and evidence-based data, often overlooked by others. To generate realistic hypothetical theories, the authors should comprehensively analyze the literature and suggest relevant and ethically sound design for future studies. They should also consider their hypotheses in the context of research and publication ethics norms acceptable for their target journals. The journal editors aiming to diversify their portfolio by maintaining and introducing hypotheses section are in a position to upgrade guidelines for related articles by pointing to general and specific analyses of the subject, preferred study designs to test hypotheses, and ethical implications. The latter is closely related to specifics of hypotheses. For example, editorial recommendations to outline benefits and risks of a new laboratory test or therapy may result in a more balanced article and minimize associated risks afterwards.

Not all scientific hypotheses have immediate positive effects. Some, if not most, are never tested in properly designed research studies and never cited in credible and indexed publication outlets. Hypotheses in specialized scientific fields, particularly those hardly understandable for nonexperts, lose their attractiveness for increasingly interdisciplinary audience. The authors' honest analysis of the benefits and limitations of their hypotheses and concerted efforts of all stakeholders in science communication to initiate public discussion on widely visible platforms and social media may reveal rational points and caveats of the new ideas.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Gasparyan AY, Yessirkepov M, Kitas GD.
• Methodology: Gasparyan AY, Mukanova U, Ayvazyan L.
• Writing - original draft: Gasparyan AY, Ayvazyan L, Yessirkepov M.
• Writing - review & editing: Gasparyan AY, Yessirkepov M, Mukanova U, Kitas GD.

5 Characteristics of a Good Hypothesis: A Guide for Researchers

• by Brian Thomas
• October 10, 2023

Are you a curious soul, always seeking answers to the whys and hows of the world? As a researcher, formulating a hypothesis is a crucial first step towards unraveling the mysteries of your study. A well-crafted hypothesis not only guides your research but also lays the foundation for drawing valid conclusions. But what exactly makes a hypothesis a good one? In this blog post, we will explore the five key characteristics of a good hypothesis that every researcher should know.

Here, we will delve into the world of hypotheses, covering everything from their types in research to understanding if they can be proven true. Whether you’re a seasoned researcher or just starting out, this blog post will provide valuable insights on how to craft a sound hypothesis for your study. So let’s dive in and uncover the secrets to formulating a hypothesis that stands strong amidst the scientific rigor!

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5 Characteristics of a Good Hypothesis

Clear and specific.

A good hypothesis is like a GPS that guides you to the right destination. It needs to be clear and specific so that you know exactly what you’re testing. Avoid vague statements or general ideas. Instead, focus on crafting a hypothesis that clearly states the relationship between variables and the expected outcome. Clarity is key, my friend!

Testable and Falsifiable

A hypothesis might sound great in theory, but if you can’t test it or prove it wrong, then it’s like chasing unicorns. A good hypothesis should be testable and falsifiable – meaning there should be a way to gather evidence to support or refute it. Don’t be afraid to challenge your hypothesis and put it to the test. Only when it can be proven false can it truly be considered a good hypothesis.

Based on Existing Knowledge

Imagine trying to build a Lego tower without any Lego bricks. That’s what it’s like to come up with a hypothesis that has no basis in existing knowledge. A good hypothesis is grounded in previous research, theories, or observations. It shows that you’ve done your homework and understand the current state of knowledge in your field. So, put on your research hat and gather those building blocks for a solid hypothesis!

Specific Predictions

No, we’re not talking about crystal ball predictions or psychic abilities here. A good hypothesis includes specific predictions about what you expect to happen. It’s like making an educated guess based on your understanding of the variables involved. These predictions help guide your research and give you something concrete to look for. So, put on those prediction goggles, my friend, and let’s get specific!

Relevant to the Research Question

A hypothesis is a road sign that points you in the right direction. But if it’s not relevant to your research question, then you might end up in a never-ending detour. A good hypothesis aligns with your research question and addresses the specific problem or phenomenon you’re investigating. Keep your focus on the main topic and avoid getting sidetracked by shiny distractions. Stay relevant, my friend, and you’ll find the answers you seek!

And there you have it: the five characteristics of a good hypothesis. Remember, a good hypothesis is clear, testable, based on existing knowledge, makes specific predictions, and is relevant to your research question. So go forth, my friend, and hypothesize your way to scientific discovery!

FAQs: Characteristics of a Good Hypothesis

In the realm of scientific research, a hypothesis plays a crucial role in formulating and testing ideas. A good hypothesis serves as the foundation for an experiment or study, guiding the researcher towards meaningful results. In this FAQ-style subsection, we’ll explore the characteristics of a good hypothesis, their types, formulation, and more. So let’s dive in and unravel the mysteries of hypothesis-making!

What Are Two Important Characteristics of a Good Hypothesis

A good hypothesis possesses two important characteristics:

Testability : A hypothesis must be testable to determine its validity. It should be formulated in a way that allows researchers to design and conduct experiments or gather data for analysis. For example, if we hypothesize that “drinking herbal tea reduces stress,” we can easily test it by conducting a study with a control group and a group drinking herbal tea.

Falsifiability : Falsifiability refers to the potential for a hypothesis to be proven wrong. A good hypothesis should make specific predictions that can be refuted or supported by evidence. This characteristic ensures that hypotheses are based on empirical observations rather than personal opinions. For instance, the hypothesis “all swans are white” can be falsified by discovering a single black swan.

What Are the Types of Hypothesis in Research

In research, there are three main types of hypotheses:

Null Hypothesis (H0) : The null hypothesis is a statement of no effect or relationship. It assumes that there is no significant difference between variables or no effect of a treatment. Researchers aim to reject the null hypothesis in favor of an alternative hypothesis.

Alternative Hypothesis (HA or H1) : The alternative hypothesis is the opposite of the null hypothesis. It asserts that there is a significant difference between variables or an effect of a treatment. Researchers seek evidence to support the alternative hypothesis.

Directional Hypothesis : A directional hypothesis predicts the specific direction of the relationship or difference between variables. For example, “increasing exercise duration will lead to greater weight loss.”

Can a Hypothesis Be Proven True

In scientific research, hypotheses are not proven true; they are supported or rejected based on empirical evidence . Even if a hypothesis is supported by multiple studies, new evidence could arise that contradicts it. Scientific knowledge is always subject to revision and refinement. Therefore, the goal is to gather enough evidence to either support or reject a hypothesis, rather than proving it absolutely true.

What Are the Six Parts of a Hypothesis

A hypothesis typically consists of six essential parts:

Research Question : A clear and concise question that the hypothesis seeks to answer.

Variables : Identification of the independent (manipulated) and dependent (measured) variables involved in the hypothesis.

Population : The specific group or individuals the hypothesis is concerned with.

Relationship or Comparison : The expected relationship or difference between variables, often indicated by directional terms like “more,” “less,” “higher,” or “lower.”

Predictability : A statement of the predicted outcome or result based on the relationship between variables.

Testability : The ability to design an experiment or gather data to support or reject the hypothesis.

How Do You Start a Hypothesis Sentence

When starting a hypothesis sentence, it is essential to use clear and concise language to express your ideas. A common approach is to use the phrase “If…then…” to establish the conditional relationship between variables. For example:

• If [independent variable], then [dependent variable] because [explanation of expected relationship].

This structure allows for a straightforward and logical formulation of the hypothesis.

What Are Examples of Hypotheses

Here are a few examples of well-formulated hypotheses:

If exposure to sunlight increases, then plants will grow taller because sunlight is necessary for photosynthesis.

If students receive praise for good grades, then their motivation to excel will increase because they seek recognition and approval.

If the dose of a painkiller is increased, then the relief from pain will last longer because a higher dosage has a prolonged effect.

What Are the Five Key Elements to a Good Hypothesis

A good hypothesis should include the following five key elements:

Clarity : The hypothesis should be clear and specific, leaving no room for interpretation.

Testability : It should be possible to test the hypothesis through experimentation or data collection.

Relevance : The hypothesis should be directly tied to the research question or problem being investigated.

Specificity : It must clearly state the relationship or difference between variables being studied.

Falsifiability : The hypothesis should make predictions that can be refuted or supported by empirical evidence.

What Makes a Good Hypothesis in a Research Paper

In a research paper, a good hypothesis should have the following characteristics:

Relevance : It must directly relate to the research topic and address the objectives of the study.

Clarity : The hypothesis should be concise and precisely worded to avoid confusion.

Unambiguous : It must leave no room for multiple interpretations or ambiguity.

Logic : The hypothesis should be based on rational and logical reasoning, considering existing theories and observations.

Empirical Support : Ideally, the hypothesis should be supported by prior empirical evidence or strong theoretical justifications.

Is a Hypothesis Always a Question

No, a hypothesis is not always in the form of a question. While some hypotheses can take the form of a question, others may be statements asserting a relationship or difference between variables. The form of a hypothesis depends on the research question being addressed and the researcher’s preferred style of expression.

What Are the Three Things Needed for a Good Hypothesis

For a hypothesis to be considered good, it must fulfill the following three criteria:

Testability : The hypothesis should be formulated in a way that allows for empirical testing through experimentation or data collection.

Falsifiability : It must make specific predictions that can be potentially refuted or supported by evidence.

Relevance : The hypothesis should directly address the research question or problem being investigated.

What Are the Four Components to a Good Hypothesis

A good hypothesis typically consists of four components:

Independent Variable : The variable being manipulated or controlled by the researcher.

Dependent Variable : The variable being measured or observed to determine the effect of the independent variable.

Directionality : The predicted relationship or difference between the independent and dependent variables.

Population : The specific group or individuals to which the hypothesis applies.

How Do You Formulate a Hypothesis

To formulate a hypothesis, follow these steps:

Identify the Research Topic : Clearly define the area or phenomenon you want to study.

Conduct Background Research : Review existing literature and research to gain knowledge about the topic.

Formulate a Research Question : Ask a clear and focused question that you want to answer through your hypothesis.

State the Null and Alternative Hypotheses : Develop a null hypothesis to assume no effect or relationship, and an alternative hypothesis to propose a significant effect or relationship.

Decide on Variables and Relationships : Determine the independent and dependent variables and the predicted relationship between them.

Refine and Test : Refine your hypothesis, ensuring it is clear, testable, and falsifiable. Then, design experiments or gather data to support or reject it.

What Is a Characteristic of a Hypothesis MCQ

Multiple-choice questions (MCQ) regarding the characteristics of a hypothesis often assess knowledge on the testability and falsifiability of hypotheses. They may ask about the criteria that distinguish a good hypothesis from a poor one or the importance of making specific predictions. Remember to choose answers that emphasize the empirical and testable nature of hypotheses.

What Five Criteria Must Be Satisfied for a Hypothesis to Be Scientific

For a hypothesis to be considered scientific, it must satisfy the following five criteria:

Testability : The hypothesis must be formulated in a way that allows it to be tested through experimentation or data collection.

Falsifiability : It should make specific predictions that can be potentially refuted or supported by empirical evidence.

Empirical Basis : The hypothesis should be based on empirical observations or existing theories and knowledge.

Relevance : It must directly address the research question or problem being investigated.

Objective : A scientific hypothesis should be free from personal biases or subjective opinions, focusing on objective observations and analysis.

What Are the Steps of Theory Development in Scientific Methods

In scientific methods, theory development typically involves the following steps:

Observation : Identifying a phenomenon or pattern worthy of investigation through observation or empirical data.

Formulation of a Hypothesis : Constructing a hypothesis that explains the observed phenomena or predicts a relationship between variables.

Data Collection : Gathering relevant data through experiments, surveys, observations, or other research methods.

Analysis : Analyzing the collected data to evaluate the hypothesis’s predictions and determine their validity.

Revision and Refinement : Based on the analysis, refining the hypothesis, modifying the theory, or formulating new hypotheses for further investigation.

Which of the Following Makes a Good Hypothesis

A good hypothesis is characterized by:

Testability : The ability to form experiments or gather data to support or refute the hypothesis.

Falsifiability : The potential for the hypothesis’s predictions to be proven wrong based on empirical evidence.

Clarity : A clear and concise statement or question that leaves no room for ambiguity.

Relevancy : Directly addressing the research question or problem at hand.

Remember, it is important to select the option that encompasses all these characteristics.

What Are the Characteristics of a Good Hypothesis

A good hypothesis possesses several characteristics, such as:

Testability : It should allow for empirical testing through experiments or data collection.

Falsifiability : The hypothesis should make specific predictions that can be potentially refuted or supported by evidence.

Clarity : It must be clearly and precisely formulated, leaving no room for ambiguity or multiple interpretations.

Relevance : The hypothesis should directly relate to the research question or problem being investigated.

What Is the Five-Step p-value Approach to Hypothesis Testing

The five-step p-value approach is a commonly used framework for hypothesis testing:

Step 1: Formulating the Hypotheses : The null hypothesis (H0) assumes no effect or relationship, while the alternative hypothesis (HA) proposes a significant effect or relationship.

Step 2: Setting the Significance Level : Decide on the level of significance (α), which represents the probability of rejecting the null hypothesis when it is true. The commonly used level is 0.05 (5%).

Step 3: Collecting Data and Performing the Test : Acquire and analyze the data, calculating the test statistic and the corresponding p-value.

Step 4: Comparing the p-value with the Significance Level : If the p-value is less than the significance level (α), reject the null hypothesis. Otherwise, fail to reject the null hypothesis.

Step 5: Drawing Conclusions : Based on the comparison in Step 4, interpret the results and draw conclusions about the hypothesis.

What Are the Stages of Hypothesis

The stages of hypothesis generally include:

Observation : Identifying a pattern, phenomenon, or research question that warrants investigation.

Formulation : Developing a hypothesis that explains or predicts the relationship or difference between variables.

Testing : Collecting data, designing experiments, or conducting studies to gather evidence supporting or refuting the hypothesis.

Analysis : Assessing the collected data to determine whether the results support or reject the hypothesis.

Conclusion : Drawing conclusions based on the analysis and making further iterations, refinements, or new hypotheses for future research.

What Is a Characteristic of a Good Hypothesis

A characteristic of a good hypothesis is its ability to make specific predictions about the relationship or difference between variables. Good hypotheses avoid vague statements and clearly articulate the expected outcomes. By doing so, researchers can design experiments or gather data that directly test the predictions, leading to meaningful results.

How Do You Write a Good Hypothesis Example

To write a good hypothesis example, follow these guidelines:

If possible, use the “If…then…” format to express a conditional relationship between variables.

Be clear and concise in stating the variables involved, the predicted relationship, and the expected outcome.

Ensure the hypothesis is testable, meaning it can be evaluated through experiments or data collection.

For instance, consider the following example:

If students study for longer periods of time, then their test scores will improve because increased study time allows for better retention of information and increased proficiency.

What Is the Difference Between Hypothesis and Hypotheses

The main difference between a hypothesis and hypotheses lies in their grammatical number. A hypothesis refers to a single statement or proposition that is formulated to explain or predict the relationship between variables. On the other hand, hypotheses is the plural form of the term hypothesis, commonly used when multiple statements or propositions are proposed and tested simultaneously.

What Is a Good Hypothesis Statement

A good hypothesis statement exhibits the following qualities:

Clarity : It is written in clear and concise language, leaving no room for confusion or ambiguity.

Testability : The hypothesis should be formulated in a way that enables testing through experiments or data collection.

Specificity : It must clearly state the predicted relationship or difference between variables.

By adhering to these criteria, a good hypothesis statement guides research efforts effectively.

What Is Not a Characteristic of a Good Hypothesis

A characteristic that does not align with a good hypothesis is subjectivity . A hypothesis should be objective, based on empirical observations or existing theories, and free from personal bias. While personal interpretations and opinions can inspire the formulation of a hypothesis, it must ultimately rely on objective observations and be open to empirical testing.

By now, you’ve gained insights into the characteristics of a good hypothesis, including testability, falsifiability, clarity,

• characteristics
• falsifiable
• good hypothesis
• hypothesis testing
• null hypothesis
• observations
• scientific rigor

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What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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How to Write a Research Hypothesis: Good & Bad Examples

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Bad Hypothesis Examples

Tips for Writing a Research Hypothesis

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

Perfect Your Manuscript With Professional Editing

Now that you know how to write a strong research hypothesis for your research paper, you might be interested in our free AI Proofreader , Wordvice AI, which finds and fixes errors in grammar, punctuation, and word choice in academic texts. Or if you are interested in human proofreading , check out our English editing services , including research paper editing and manuscript editing .

On the Wordvice academic resources website , you can also find many more articles and other resources that can help you with writing the other parts of your research paper , with making a research paper outline before you put everything together, or with writing an effective cover letter once you are ready to submit.

Developing a Hypothesis

Two girls exploring plant life in the woods (Christine Glade, iStockphoto)

How does this align with my curriculum?

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Learn what makes a good hypothesis, and how to develop one.

Developing a Scientific Hypothesis

After identifying a  testable question , it is important for students to research and or/review what they already know about the scientific principles involved in their experimental inquiries. After that, and before conducting the inquiry through testing and observation, students need to develop a scientific  hypothesis .

Is a hypothesis the same as a guess?

The short answer is no! Anyone can make a guess about anything. Guesses are not generally based on knowledge, but rather are rough estimates that people give when they don’t know the answer to a question. A scientific hypothesis, on the other hand, is not only based on prior knowledge and experiences but also on known factual information obtained through research.

Misconception Alert Like making an estimate in math, a hypothesis should be written before doing an inquiry, not after!

Is a hypothesis the same as a prediction? 

Again, the answer is no, although the distinction between these two terms is not always clear. A  prediction  is an estimate or forecast about something that might happen or the way that something will be based on prior knowledge and experience and known facts (e.g., I predict it will rain tomorrow, I predict that my plant will have two seed leaves, etc.).

Shown is a colour photograph of a boat near a beach in the rain. The camera lens is sprinkled with rain drops. These are in sharp focus, in the foreground. Most of the rest of the photograph is out of focus in the background.  There is a strip of dark green grass along the bottom of the frame. Above that is a strip of beige sand. The water is shaded from light grey at the bottom to medium grey at the top. A dark green hill rises up behind the water, to the left. The sky is mottled with grey, white and blueish grey clouds. A small, yellow wooden rowboat is in sharp focus. It is moored to the shore with a white rope, next to a round, orange float.

Like a prediction, a hypothesis forecasts what might happen, but a hypothesis goes beyond a prediction. It includes not only what might happen, but why something might happen. In other words, it explains the relationship between variables. The most significant difference between a prediction and an hypothesis is that a hypothesis is intended to lead to a testable investigation, whereas a prediction is not.

To put it in a different way, a prediction is an estimate of an end result (e.g., I predict that the plant will be tall) whereas a hypothesis is a statement that attempts to explain a phenomena by relating cause and effect (e.g., if we give plants more water, then they will grow taller).

Shown is a colour photograph of water falling from a blue watering can onto a pink flowering plant.  The frame is filled with green foliage. In the background, out of focus, is a row of pink, flowering plants in boxes along a wooden railing. In the foreground, a gloved hand tips a large, cornflower blue watering can over the first plant.

Misconception Alert Not every inquiry lends itself to the testing of a hypothesis. Many inquiries involve research questions that ask if relationships exist among variables or involve situations where testing is not possible, such as population inquiries, historical inquiries, etc. For example, you could never test a hypothesis about which type of food a given dinosaur preferred to eat!

Shown is a colour photograph of miniature plastic dinosaurs gathered around a piece of broccoli that resembles a tree.  A piece of broccoli stands upright in the middle of the photograph. It has a long, pale green stem and a full, dark green floret. It looks like a green tree with branches and tiny leaves. Five toy dinosaurs have been placed around the broccoli so they look like they're snacking it. They are a little bit shorter than the broccoli, so their mouths reach the bushiest parts of it.  The dinosaur in the foreground is dark reddish brown with scaly-looking skin and tiny arms. Behind it, a dark green dinosaur with a long neck stretches to the low branches. In the background, a dark brown triceratops looks on. The long neck of a black dinosaur reaches in from the left, to get the higher leaves. On the far left, a bright yellow dinosaur is about to join the meal.

How do you develop a scientific hypothesis?

In order to develop a hypothesis, one should have:

• A good  testable question
• Understanding of the dependent, independent and control  variables  of interest
• Some prior knowledge, such as from observations and research
• Thoughts about how the inquiry could be done (the method)

For example, students may begin with the question:

How does the duration of light exposure affect the surface area of tomato plant leaves?

The variables are:

• Independent = duration of light
• Dependent = surface area of plant leaves
• Controlled = water, soil, seed source, etc.

How then do we formulate a hypothesis from this testable question? A good hypothesis tends to follow the format:

If  we do/change this 

Then  this will happen/be observed, because  we know this., if  these changes are made to a certain independent variable,, then  will we observe a change in a specific dependent variable, because  of our prior knowledge and research..

In the example above, the students have identified that they are interested in exploring how the duration of light affects plants, perhaps exposing plants from the same batch of seeds to light for different numbers of hours (e.g., one hour, two hours, etc.). Knowing that plants need light to grow (from prior knowledge or research), then they may hypothesize that the leaves of a plant may be larger given a longer exposure to light. Knowing all of this, their hypothesis might be:

If  we expose plants to a greater number of hours of light,  then  the surface area of the tomato plant leaves will be larger  because  light affects plant growth.

What makes for a good hypothesis.

A good hypothesis is:

• A statement  The hypothesis is  not  the same as the testable question. The hypothesis is a tentative explanation of what is thought will happen during the inquiry.
• Testable  What is changed (independent variable) and what is affected by the change (dependent variable) should be measurable and observable.
• Falsifiable  A good hypothesis can be either supported or shown to be false by the data collected.
• Clear.  It should be obvious what will be tested, how it will be tested (what will be measured to prove or disprove the hypothesis), and what is expected to happen.

A good question and hypothesis should also help students find answers that are not obvious to them or generally known. For example, most students will know that if you do not water a plant, it will die, so developing a hypothesis such as:

If  we stop watering our plants  then  the plants will die  because  plants need water in order to live.

is overly simplistic and will not help students expand their knowledge. A good experimental inquiry will help students discover things they do not already know.

Misconception Alert The goal of a hypothesis is NOT for a student to be “right.” Having evidence that shows a hypothesis to be false is just as important as having evidence that shows it to be true. A hypothesis is NOT something you prove – it is something you test!

How to develop a Tomatosphere™ hypothesis 

In the Seed Investigation, a testable question is provided to the students:

How does exposure to the space environment or space-like conditions affect the number of tomato seeds that germinate?

In the  variables  section, the dependent and independent variables were identified.

Independent variable :  Seed treatment – Some seeds have been to space or are exposed to space-like conditions in years when seeds do not go to space, while some seeds have not been to space or exposed to space-like conditions.

Dependent variable : Number of seeds that germinate.

What is not provided to the students is a hypothesis to follow from this question. Using the “if…..then…because….” format, have the students develop their hypotheses for the Tomatosphere™ testable question. For example:

If  tomato plant seeds are exposed to the conditions of space,  then  fewer ‘space’ seeds will germinate than non- ‘space’ seeds  because  radiation levels found in space may damage cells in the seeds.

This is not the only possible hypothesis, but it shows some understanding of how plants might be affected by space conditions (e.g., radiation affecting DNA in cells, microgravity affecting growth, etc.) which might be derived from prior knowledge or research.

Is this a good hypothesis? Yes

• It is a statement.
• It is testable.  What is changed (being in space or not) and what is affected by the change (number of seeds germinated) can be measured and observed.
• It is falsifiable.  The student can use the data collected to be able to decide if it supports their hypothesis or if it shows the hypothesis is false (statement is false – more ‘space’ seeds germinate or the germination rate is the same).
• It is clear.  It should be obvious what will be tested (seed germination), how it will be tested (seeds are grown to the point of germination), and what is expected to happen (fewer space seeds will germinate).

To assist with practicing writing a hypothesis, students could be provided with a checklist, such as this one, also available as a [ Google doc ] and [ PDF ].

Writing a Strong Hypothesis Checklist

Hypothesis is a statement that correctly follows the format:

"If _____ then ______ because _________

Hypothesis relates to the Testable Question

Hypothesis makes sense (based on observations and/or research)

Hypothesis can be falsified

Hypothesis includes a cause and effect relationship

Hypothesis could be tested with measurements

Hypothesis is easy to understand

Guided Practice

Have students read the following statements and determine if these are good, okay, or poor hypotheses and why.

Have students use the  Writing a Strong Hypothesis Checklist  for creating a Tomatosphere™ or other hypothesis.

Why is this a good hypothesis? ✓  It is a  statement  that follows the “if….then…because” format. ✓ It is testable.  What is changed (red light vs. green light) and what is affected by the change (size of leaves) is measurable and observable. ✓ It is falsifiable.  It can be supported by evidence (statement is true – leaves will be bigger, statement is untrue – leaves will be smaller or the same size). ✓ It is clear.  It is obvious what will be tested (two colours of light), how it will be tested (at six weeks of age the plant leaves will be measured), and what is expected to happen (plants grown in red light will have bigger leaves). B): 

Why is this a poor hypothesis? ✓  It is a  statement  that follows the “if….then…because” format. ✓ It is not testable.  The variables are very vague. What are the classroom conditions compared to the outdoor conditions? Is the interest in soil? Light? Temperature? What kinds of plants will be grown. ✗ It is not falsifiable.  It would be difficult to support or falsify with evidence because it is vague. ✗ It is not clear.  It is not obvious what will be tested (Soil? Temperature? Light?), how it will be tested and what is expected to happen (what does “better” mean? Taller? Bigger leaves? Flower sooner?). Have students work on changing this vague hypothesis into a more specific one by identifying variables to explore. C): 

Why is this just an “okay” hypothesis? ✓  It is a  statement  that follows the “if….then…because” format. ✗ It is somewhat testable.  What is changed (sugar water vs. regular water) is clear, but what is affected by the change (“better”) is vague. Will the plants be taller? Grow faster? ✗ It is not falsifiable.  It would be difficult to support or falsify with evidence because the “better” is vague. ✗ It is somewhat clear.   It is obvious what will be tested (maple syrup being added to the water) and how it will be tested, but what is expected to happen is not clear (what does “better” mean? Taller? Bigger leaves? Flower sooner?). Have students work on changing this somewhat vague hypothesis into a more specific one by identifying a dependent variable.

A Strong Hypothesis - Science Buddies  (2010) This blog post by Science Buddies explains the parts of a good hypothesis, and the role a hypothesis plays in the scientific process.

Theory vs. Hypothesis vs. Law… Explained!  (2015) This video (7:11 min.) from PBS Studios Be Smart explains how these words mean something totally different in science than in everyday speech, and how they all help us understand how the universe works.

Misconceptions about Science This page by Understanding Science at UC Berkeley gives a thorough definition of the word hypothesis, in a scientific context, as opposed to everyday language.

What is a Scientific Hypothesis?  (2022) This article by Alina Bradford at Live Science discusses what makes a hypothesis testable, the different types of hypotheses, and hypothesis vs. theory.

Writing a Hypothesis  (2013) This video (4:58 min.) by mreppsclassroom explains the purpose of a hypothesis and how to construct one.

Related Topics

Scientific Hypothesis

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Embarking on a scientific journey requires hypotheses that challenge, inspire, and guide your inquiries. The essence of any research, a well-framed hypothesis, serves as the compass that directs experiments and Thesis statement analysis. Dive into this comprehensive guide that unfolds a rich tapestry of scientific hypothesis statement examples, elucidates the steps to craft your own, and shares invaluable tips to ensure precision and relevance in your exploratory endeavors.

What is a good Scientific hypothesis statement example?

A good scientific hypothesis statement should be clear, concise, and testable. It should predict a cause-and-effect relationship between two or more variables. For instance: “If soil moisture levels decrease, then plant growth rates will also decrease.”

What is an example of a scientific hypothesis statement?

Consider a researcher studying the effects of sunlight on plant growth. The hypothesis might be: “If a plant is exposed to increased hours of sunlight, then it will grow taller than a plant that receives fewer hours of sunlight.” This Simple hypothesis sets a clear expectation (plant growth) based on a specific condition (hours of sunlight) and is easily testable through experimentation.

100 Scientific Statement Examples

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Scientific thesis statements serve as the backbone of research, setting forth clear and testable claims about phenomena. These assertions provide researchers with a focused direction and help them communicate their study’s core intent. Below are captivating examples spanning diverse scientific disciplines.

• Ecology: Increased urbanization will lead to a decrease in biodiversity in metropolitan areas.
• Genetics: Alterations in the BRCA1 gene increase susceptibility to breast cancer in women.
• Astronomy: Planets located within the habitable zone of their star system are more likely to contain traces of water.
• Chemistry: Increasing the temperature of a reaction will increase the rate at which that reaction occurs, up to a point.
• Physics: In the absence of air resistance, all objects fall at the same rate irrespective of their mass.
• Marine Biology: Coral bleaching events are directly correlated with rising sea temperatures.
• Meteorology: The increase in global temperatures has accelerated the melting rate of polar ice caps.
• Neuroscience: Chronic exposure to stress can lead to irreversible damage in the hippocampus of the brain.
• Geology: Tectonic activity along the Pacific Ring of Fire will increase the likelihood of major earthquakes in the region.
• Botany: Plants grown in higher concentrations of carbon dioxide will have faster photosynthesis rates.
• Zoology: Animals that have more intricate mating dances have a higher likelihood of attracting a mate.
• Microbiology: Bacterial resistance to antibiotics increases with the overuse of these medications.
• Biochemistry: Enzymes lose their effectiveness when subjected to temperatures beyond their optimal range.
• Psychology: Exposure to violent video games correlates with aggressive behavior in adolescents.
• Anthropology: Ancient human migration patterns can be traced through the study of mitochondrial DNA.
• Pharmacology: The introduction of Drug X will reduce symptoms of depression more effectively than currently prescribed antidepressants.
• Climatology: An increase in greenhouse gas emissions directly correlates with rising global temperatures.
• Paleontology: The mass extinction event at the end of the Cretaceous period was caused by a meteor impact.
• Mathematics: Prime numbers greater than 2 are always odd numbers.
• Biophysics: Cellular osmosis rates are influenced by the concentration gradient of solute molecules.
• Ornithology: Birds that migrate longer distances have more streamlined body shapes to enhance aerodynamic efficiency.
• Immunology: Vaccinating children against measles will drastically reduce the occurrence of the disease in the general population.
• Nanotechnology: Nanoparticles can be effectively used to target and treat specific cancer cells.
• Environmental Science: The increase in plastic waste in oceans is negatively impacting marine life.
• Molecular Biology: The transcription rate of DNA into RNA is influenced by specific protein regulators.
• Entomology: Insect species that undergo metamorphosis have a higher survival rate than those that don’t.
• Genomics: Identifying specific gene markers can help predict susceptibility to Type 2 Diabetes.
• Agronomy: Crop yields improve with the rotation of specific plant species.
• Astrophysics: Black holes can be identified by observing the radiation emitted at their event horizon.
• Material Science: The tensile strength of a metal increases with the addition of specific alloys.
• Toxicology: Prolonged exposure to pollutant X increases the risk of respiratory diseases in urban dwellers.
• Endocrinology: Hormone imbalances can lead to metabolic syndromes in mammals.
• Space Science: The existence of exoplanets around binary star systems suggests diverse planetary formation processes.
• Physiology: High-intensity interval training (HIIT) increases metabolic rates more significantly than steady-state cardio exercises.
• Quantum Mechanics: Particles can display both wave-like and particle-like behavior under specific observational conditions.
• Pedology: Soil health directly influences the nutritional quality of food crops grown in that soil.
• Mycology: Fungi play a critical role in forest ecosystems by decomposing organic matter and forming symbiotic relationships with trees.
• Virology: Viruses that mutate rapidly pose higher challenges for vaccine development.
• Hydrology: Urban development and deforestation increase the risk of flash floods due to reduced soil absorption capacities.
• Structural Biology: The 3D arrangement of proteins influences their functionality and interaction with other molecules.
• Thermodynamics: An isolated system will always move towards a state of maximum entropy.
• Arachnology: Spider silk’s tensile strength can rival that of steel when adjusted for thickness.
• Paleobotany: The presence of certain ancient pollen types can indicate past climatic conditions of a region.
• Oceanography: Ocean acidification is causing significant disruptions to marine food chains.
• Spectroscopy: Molecules can be identified based on the absorption and emission spectra of light they produce.
• Cytology: Cell division rates can be influenced by the surrounding micro-environment and external growth factors.
• Ethology: Animal behaviors, such as nesting and migration, often correlate with seasonal changes.
• Optics: Light’s behavior changes when passing through materials with different refractive indices.
• Volcanology: Certain gas emissions from volcanoes can serve as early indicators of potential eruptions.
• Bacteriology: Beneficial gut bacteria play a role in digestion and overall human health.
• Nephrology: High sodium intake correlates with increased risk factors for chronic kidney diseases.
• Chronobiology: The human circadian rhythm influences sleep patterns, alertness, and hormone production.
• Rheology: The viscosity of a fluid changes under different temperatures and pressures.
• Aerodynamics: Wing shapes in aircraft influence fuel efficiency and maneuverability.
• Seismology: Earthquake aftershocks can be predicted based on the magnitude of the primary quake.
• Mineralogy: Specific minerals can be identified by their unique crystalline structures and optical properties.
• Pathology: The progression of disease Y is accelerated by genetic predisposition.
• Cosmology: The observed redshift of distant galaxies supports the theory of the expanding universe.
• Dermatology: UV exposure is the primary factor leading to premature skin aging.
• Epidemiology: Vaccination rates correlate inversely with the incidence of infectious diseases in a population.
• Gastroenterology: Diets high in processed sugars correlate with an increased risk of gastrointestinal disorders.
• Forestry: Old growth forests store more carbon per acre than younger, reforested areas.
• Astrobiology: The presence of methane on Mars might suggest microbial life below its surface.
• Hematology: Individuals with blood type O are universal donors for blood transfusions.
• Gerontology: Caloric restriction can extend lifespan in certain organisms.
• Ichthyology: Overfishing in a specific region leads to a decline in the diversity of marine species.
• Limnology: Freshwater lakes with high nutrient runoffs are more susceptible to algal blooms.
• Mammalogy: The echolocation frequency of bats is adapted to their specific prey type.
• Nuclear Physics: The stability of an atomic nucleus depends on the ratio of its protons to neutrons.
• Odonatology: Dragonfly wing patterns play a significant role in mate selection and territorial disputes.
• Petrology: The mineral composition of igneous rocks can indicate the conditions under which they formed.
• Radiology: Modern MRI techniques can detect neural anomalies leading to specific cognitive disorders.
• Statistical Physics: The behavior of macroscopic systems can be predicted by understanding the statistical behaviors of its microscopic constituents.
• Urology: High fluid intake can reduce the risk of kidney stone formation.
• Xenobiology: (Hypothetical) If life exists on exoplanets, it might not be carbon-based, leading to diverse biochemistries.
• Zymology: The fermentation rate of yeast is influenced by sugar concentration and ambient temperature.
• Dendrology: Tree ring patterns can serve as indicators of past climatic conditions.
• Electrophysiology: Neuronal firing rates can be modulated by external electrical stimulation.
• Fossil Fuels: The over-reliance on fossil fuels directly correlates with increased atmospheric CO2 levels.
• Herpetology: Amphibian populations are declining globally due to a combination of habitat loss, pollution, and fungal diseases.
• Kinesiology: Proper biomechanics during physical activities can reduce the risk of injury.
• Lepidopterology: Moth species that mimic unpalatable butterfly species have higher survival rates against predators.
• Mycorrhizae: Fungal and plant root symbiotic relationships enhance nutrient absorption.
• Neuropharmacology: Drug Z shows potential in slowing the progression of Alzheimer’s disease.
• Ornithological Behavior: Birds adjust their migratory patterns in response to changes in food availability.
• Paleoecology: Fossilized pollen and spores can provide clues about ancient ecosystems and climate conditions.
• Quantum Biology: Quantum effects might play a role in efficient energy transfer during photosynthesis.
• Raptor Biology: Urban environments affect the hunting strategies of birds of prey.
• Symbiosis: Mutualistic relationships between species X and Y lead to a more efficient nutrient cycle.
• Tectonics: The movement of tectonic plates influences global climatic patterns over geologic time scales.
• Vertebrate Zoology: The skeletal adaptations of burrowing animals provide increased strength and flexibility for underground movement.
• Weather Patterns: La Niña conditions in the Pacific Ocean correlate with increased rainfall in the Southwestern United States.
• X-ray Crystallography: Protein structures determined through X-ray diffraction techniques provide insights into molecular interactions and functionality.
• Yeast Genetics: Manipulating specific genes in yeast can enhance their fermentation efficiency, impacting biofuel production.
• Zoonotic Diseases: Human encroachment into wild habitats increases the risk of zoonotic disease transmission.
• Agroforestry: Integrating trees into farmlands enhances biodiversity, improves soil quality, and can increase crop yields.
• Bioinformatics: Computational tools in analyzing DNA sequences can predict potential functions of unknown genes.
• Climatology: The ongoing rise in global average temperatures suggests a significant anthropogenic influence on the climate.
• Dermatophytosis: Fungi causing skin infections in humans show increasing resistance to traditional antifungal treatments.
• Ecotourism: Sustainable ecotourism practices can aid in conservation efforts and boost local economies.

Scientific Hypothesis Statement Examples for Research

Scientific hypothesis for research serve as tentative explanations for specific phenomena, which can be tested through experiments or observations. They’re foundational in guiding the direction of scientific inquiry.

• Ozone Depletion: The depletion of ozone in Earth’s atmosphere is majorly contributed by human-made chemicals like CFCs.
• Plant Growth: The rate of plant growth in a hydroponic system is faster compared to traditional soil gardening.
• Aerodynamics: Modified wingtip designs reduce drag and improve fuel efficiency in aircraft.
• Brain Plasticity: Regular cognitive exercises can slow the degenerative processes in aging brains.
• Marine Biology: Coral reefs that experience frequent temperature fluctuations are more resilient to coral bleaching events.
• Chemistry: The rate of chemical reaction X increases with a rise in temperature up to a certain point.
• Geology: Regions with more frequent earthquakes have a thinner lithosphere.
• Endocrinology: Consuming foods high in sugar leads to a rapid spike in insulin levels.
• Environmental Science: Urban areas with more green spaces have lower levels of air pollution.
• Quantum Mechanics: Particle behavior at the quantum level is influenced by the act of observation.

Scientific Investigation Hypothesis Statement Examples

Hypotheses in scientific investigations are proposed explanations or predictions that are directly testable, usually through experiments or special observational techniques.

• Astronomy: The brightness variation of star X is due to the presence of a large exoplanet.
• Microbiology: The presence of bacteria Y in water sources correlates with the onset of disease Z in communities.
• Genetics: Gene A in fruit flies is responsible for wing color variation.
• Neurology: The prolonged use of digital devices causes changes in the sleep patterns of adolescents.
• Ecology: Introduction of a predator in ecosystem B will reduce the population of herbivores.
• Physics: Materials with a higher rate of thermal conductivity cool down faster when exposed to the same conditions.
• Psychology: Exposure to nature reduces levels of stress and anxiety in adults.
• Volcanology: Active volcanoes with higher silica content in their magma are more likely to erupt explosively.
• Anthropology: Early human migrations were influenced by climate changes.
• Botany: Plants exposed to music grow faster than those that aren’t.

Scientific Null Hypothesis Statement Examples

Null hypothesis assert that there is no significant difference or effect, serving as a default stance in research until evidence suggests otherwise.

• Medicine: Treatment A has no significant effect on the recovery rate of patients compared to the placebo.
• Behavioral Science: There is no measurable difference in test scores between students taught with method X versus method Y.
• Genetics: There is no relationship between gene B and the trait C in species D.
• Climatology: Changes in global temperature do not depend on the amount of carbon dioxide in the atmosphere.
• Pharmacology: Drug E does not significantly alter blood pressure levels more than the standard medication.
• Zoology: There is no difference in the lifespans of species F in the wild versus in captivity.
• Agriculture: Fertilizer G doesn’t increase crop yields more than the traditional fertilizer.
• Physics: Changing the material of wire H does not affect its electrical conductivity.
• Marine Science: The presence of pollutant I has no significant impact on fish reproduction rates.
• Paleontology: The morphology of fossil J is not influenced by the environment it once inhabited.

Testable Scientific Hypothesis Statement Examples

A testable hypothesis is an actionable statement that can be examined and evaluated through empirical means, ensuring clarity and precision in scientific endeavors.

• Meteorology: Increased cloud cover over region K results in decreased daytime temperatures.
• Physiology: Regular exercise increases bone density in adults over the age of 50.
• Geography: River meandering intensity is directly related to the gradient of the terrain.
• Chemical Engineering: Catalyst L enhances the efficiency of reaction M by at least 20%.
• Ornithology: Birds of species N change their migration patterns due to shifts in global temperature.
• Material Science: Alloy O has twice the tensile strength of its primary metal component.
• Sociology: Communities with more recreational areas report higher levels of general well-being.
• Optics: Lens P refracts light at a different angle than lens Q, affecting image clarity.
• Forensics: The presence of substance R is indicative of a specific cause of death.
• Endocrinology: Hormone S levels are directly proportional to the intensity of emotion T.

Scientific Hypothesis Statement Examples for Action Research

In action research, hypotheses often focus on interventions and their outcomes, allowing for iterative improvements in practice based on findings.

• Education: Implementing multimedia tools in classroom U enhances student engagement and understanding.
• Urban Planning: Introducing green corridors in city V reduces the urban heat island effect.
• Healthcare: Incorporating mindfulness exercises in daily routines reduces burnout rates among nurses.
• Agriculture: Using natural predator W reduces pest populations without affecting crop health.
• Community Development: Local art initiatives boost community morale and reduce vandalism rates.
• Business: Employee training program X increases sales by at least 15% in the subsequent quarter.
• Conservation: Implementing recycling program Y in city Z increases waste diversion by 30%.
• Transportation: Carpool initiatives reduce traffic congestion during peak hours.
• Mental Health: Cognitive-behavioral therapy techniques reduce symptom severity in patients with phobias.
• Technology: Introduction of software A in company B enhances workflow efficiency by 25%.

Alternative Hypothesis Statement Examples in Scientific Study

The alternative hypothesis posits a potential relationship or effect, opposing the null hypothesis and indicating a significant result in research.

• Oceanography: Deep-sea mining significantly affects the biodiversity of marine ecosystems.
• Epidemiology: Vaccination rates are inversely related to the incidence of disease C in population D.
• Astronomy: The luminosity of star E is influenced by the presence of nearby celestial bodies.
• Toxicology: Exposure to chemical F at concentration G leads to health complications H.
• Microbiology: The growth rate of bacteria I is inhibited by the presence of antibiotic J.
• Hydrology: River K’s flow rate is influenced by the lunar cycle.
• Seismology: Tectonic activity L is related to the occurrence of supermoons.
• Anthropology: Cultural practices M in tribe N evolved due to environmental pressures O.
• Quantum Physics: The behavior of particle P is determined by the presence of field Q.
• Biochemistry: The activity of enzyme R is enhanced in the presence of compound S.

Scientific Development Hypothesis Statement Examples

These hypotheses address the developmental processes in various fields of science, focusing on growth, evolution, and stages of progression.

• Embryology: Exposure to substance T during the embryonic stage leads to developmental anomalies in species U.
• Evolution: Species V evolved specific traits in response to predation pressures.
• Cognitive Science: Neural connections in the brain’s W region develop faster in children exposed to bilingual environments.
• Plant Science: Plant X’s growth phases are influenced by light duration and intensity.
• Endocrinology: The development of gland Y in adolescents is influenced by nutritional factors.
• Neuroscience: Neuron type Z in the brain develops in response to sensory stimuli during early childhood.
• Genetics: Certain genetic markers indicate a predisposition to developmental disorders A.
• Palaeontology: Dinosaur species B developed feathers for thermoregulation before they were used for flight.
• Pharmacology: The development of drug resistance in bacteria C is influenced by the misuse of antibiotics.
• Sociology: Social structures D in ancient civilizations developed in response to geographic and climatic challenges.

What is a hypothesis in the scientific method?

A s cience hypothesis is a fundamental component of the scientific method, serving as a bridge between the formulation of research questions and the execution of experiments or observations. It is a proposed explanation or prediction about a specific phenomenon, based on prior knowledge, observation, or reasoning, which can be tested and either confirmed or refuted.

The role of a hypothesis in the scientific method can be broken down into several key points:

• Foundation for Research: It provides a clear focus and direction for the research by stipulating what the researcher expects to find or verify.
• Testability: For a hypothesis to be considered scientific, it must be testable through empirical methods (observations or experiments).
• Falsifiability: A scientific hypothesis must also be falsifiable, meaning there should be potential outcomes of the research that would prove the hypothesis wrong. This is a critical aspect of the scientific method, ensuring that hypotheses are not merely speculative.
• Predictive Power: A hypothesis often predicts specific outcomes, allowing for the design of experiments to test these predictions.
• Refinement of Knowledge: Once a hypothesis is tested, it can either be supported, refuted, or require modification, contributing to the evolving body of scientific knowledge.

How do you write a hypothesis statement for Scientific Research – Step by Step Guide

• Identify the Research Question: Before you can write a hypothesis, you need to pinpoint what you’re trying to find out. This could arise from observations, literature reviews, or gaps in current knowledge.
• Conduct Preliminary Research: Get familiar with existing literature and studies on the topic to ensure your hypothesis is novel and relevant.
• Determine the Variables: Identify the independent variable (what you will change) and the dependent variable (what you will observe or measure).
• Formulate the Hypothesis: Write a clear, concise statement that predicts the relationship or effect between the variables. Ensure it’s testable and falsifiable.
• Ensure Clarity: The hypothesis should be specific and unambiguous, so that anyone reading it understands your prediction.
• Check Falsifiability: Ensure there are potential outcomes that could prove your hypothesis incorrect.
• Re-evaluate and Refine: Go back to existing literature or seek peer feedback to ensure your hypothesis is sound and relevant.

Tips for Writing a Scientific Hypothesis Statement

• Be Concise: A hypothesis should be a clear and concise statement, not a question or a vague idea.
• Use Clear Language: Avoid jargon or overly complex language. The statement should be understandable to someone outside of the specific research field.
• Ensure It’s Testable: A hypothesis should make a claim that can be supported or refuted through experimentation or observation.
• Prioritize Falsifiability: While it might be tempting to craft a hypothesis that’s sure to be proven right, it’s essential that there are ways it could be proven wrong.
• Avoid Absolutes: Steer clear of words like “always” or “never” as they can make your hypothesis untestable. Instead, opt for terms that indicate a relationship or effect.
• Stay Relevant: Your hypothesis should be pertinent to the research question and reflect current scientific understanding.
• Seek Feedback: Before finalizing your hypothesis, it can be beneficial to get feedback from peers, mentors, or experts in the field.
• Be Prepared to Revise: As you delve deeper into your research, you may find that your original hypothesis needs refining or modification. This is a natural part of the scientific process.

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What Is a Hypothesis and How Do I Write One?

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Think about something strange and unexplainable in your life. Maybe you get a headache right before it rains, or maybe you think your favorite sports team wins when you wear a certain color. If you wanted to see whether these are just coincidences or scientific fact, you would form a hypothesis, then create an experiment to see whether that hypothesis is true or not.

But what is a hypothesis, anyway? If you’re not sure about what a hypothesis is--or how to test for one!--you’re in the right place. This article will teach you everything you need to know about hypotheses, including: 

• Defining the term “hypothesis” 
• Providing hypothesis examples 
• Giving you tips for how to write your own hypothesis

So let’s get started!

What Is a Hypothesis?

Merriam Webster defines a hypothesis as “an assumption or concession made for the sake of argument.” In other words, a hypothesis is an educated guess . Scientists make a reasonable assumption--or a hypothesis--then design an experiment to test whether it’s true or not. Keep in mind that in science, a hypothesis should be testable. You have to be able to design an experiment that tests your hypothesis in order for it to be valid. 

As you could assume from that statement, it’s easy to make a bad hypothesis. But when you’re holding an experiment, it’s even more important that your guesses be good...after all, you’re spending time (and maybe money!) to figure out more about your observation. That’s why we refer to a hypothesis as an educated guess--good hypotheses are based on existing data and research to make them as sound as possible.

Hypotheses are one part of what’s called the scientific method .  Every (good) experiment or study is based in the scientific method. The scientific method gives order and structure to experiments and ensures that interference from scientists or outside influences does not skew the results. It’s important that you understand the concepts of the scientific method before holding your own experiment. Though it may vary among scientists, the scientific method is generally made up of six steps (in order):

• Observation
• Asking questions
• Forming a hypothesis
• Analyze the data
• Communicate your results

You’ll notice that the hypothesis comes pretty early on when conducting an experiment. That’s because experiments work best when they’re trying to answer one specific question. And you can’t conduct an experiment until you know what you’re trying to prove!

Independent and Dependent Variables 

After doing your research, you’re ready for another important step in forming your hypothesis: identifying variables. Variables are basically any factor that could influence the outcome of your experiment . Variables have to be measurable and related to the topic being studied.

There are two types of variables:  independent variables and dependent variables. I ndependent variables remain constant . For example, age is an independent variable; it will stay the same, and researchers can look at different ages to see if it has an effect on the dependent variable. 

Speaking of dependent variables... dependent variables are subject to the influence of the independent variable , meaning that they are not constant. Let’s say you want to test whether a person’s age affects how much sleep they need. In that case, the independent variable is age (like we mentioned above), and the dependent variable is how much sleep a person gets. 

Variables will be crucial in writing your hypothesis. You need to be able to identify which variable is which, as both the independent and dependent variables will be written into your hypothesis. For instance, in a study about exercise, the independent variable might be the speed at which the respondents walk for thirty minutes, and the dependent variable would be their heart rate. In your study and in your hypothesis, you’re trying to understand the relationship between the two variables.

Elements of a Good Hypothesis

The best hypotheses start by asking the right questions . For instance, if you’ve observed that the grass is greener when it rains twice a week, you could ask what kind of grass it is, what elevation it’s at, and if the grass across the street responds to rain in the same way. Any of these questions could become the backbone of experiments to test why the grass gets greener when it rains fairly frequently.

As you’re asking more questions about your first observation, make sure you’re also making more observations . If it doesn’t rain for two weeks and the grass still looks green, that’s an important observation that could influence your hypothesis. You'll continue observing all throughout your experiment, but until the hypothesis is finalized, every observation should be noted.

Finally, you should consult secondary research before writing your hypothesis . Secondary research is comprised of results found and published by other people. You can usually find this information online or at your library. Additionally, m ake sure the research you find is credible and related to your topic. If you’re studying the correlation between rain and grass growth, it would help you to research rain patterns over the past twenty years for your county, published by a local agricultural association. You should also research the types of grass common in your area, the type of grass in your lawn, and whether anyone else has conducted experiments about your hypothesis. Also be sure you’re checking the quality of your research . Research done by a middle school student about what minerals can be found in rainwater would be less useful than an article published by a local university.

Writing Your Hypothesis

Once you’ve considered all of the factors above, you’re ready to start writing your hypothesis. Hypotheses usually take a certain form when they’re written out in a research report.

When you boil down your hypothesis statement, you are writing down your best guess and not the question at hand . This means that your statement should be written as if it is fact already, even though you are simply testing it.

The reason for this is that, after you have completed your study, you'll either accept or reject your if-then or your null hypothesis. All hypothesis testing examples should be measurable and able to be confirmed or denied. You cannot confirm a question, only a statement! 

In fact, you come up with hypothesis examples all the time! For instance, when you guess on the outcome of a basketball game, you don’t say, “Will the Miami Heat beat the Boston Celtics?” but instead, “I think the Miami Heat will beat the Boston Celtics.” You state it as if it is already true, even if it turns out you’re wrong. You do the same thing when writing your hypothesis.

Additionally, keep in mind that hypotheses can range from very specific to very broad.  These hypotheses can be specific, but if your hypothesis testing examples involve a broad range of causes and effects, your hypothesis can also be broad.  

The Two Types of Hypotheses

Now that you understand what goes into a hypothesis, it’s time to look more closely at the two most common types of hypothesis: the if-then hypothesis and the null hypothesis.

#1: If-Then Hypotheses

First of all, if-then hypotheses typically follow this formula:

If ____ happens, then ____ will happen.

The goal of this type of hypothesis is to test the causal relationship between the independent and dependent variable. It’s fairly simple, and each hypothesis can vary in how detailed it can be. We create if-then hypotheses all the time with our daily predictions. Here are some examples of hypotheses that use an if-then structure from daily life: 

• If I get enough sleep, I’ll be able to get more work done tomorrow.
• If the bus is on time, I can make it to my friend’s birthday party. 
• If I study every night this week, I’ll get a better grade on my exam. 

In each of these situations, you’re making a guess on how an independent variable (sleep, time, or studying) will affect a dependent variable (the amount of work you can do, making it to a party on time, or getting better grades). 

You may still be asking, “What is an example of a hypothesis used in scientific research?” Take one of the hypothesis examples from a real-world study on whether using technology before bed affects children’s sleep patterns. The hypothesis read s:

“We hypothesized that increased hours of tablet- and phone-based screen time at bedtime would be inversely correlated with sleep quality and child attention.”

It might not look like it, but this is an if-then statement. The researchers basically said, “If children have more screen usage at bedtime, then their quality of sleep and attention will be worse.” The sleep quality and attention are the dependent variables and the screen usage is the independent variable. (Usually, the independent variable comes after the “if” and the dependent variable comes after the “then,” as it is the independent variable that affects the dependent variable.) This is an excellent example of how flexible hypothesis statements can be, as long as the general idea of “if-then” and the independent and dependent variables are present.

#2: Null Hypotheses

Your if-then hypothesis is not the only one needed to complete a successful experiment, however. You also need a null hypothesis to test it against. In its most basic form, the null hypothesis is the opposite of your if-then hypothesis . When you write your null hypothesis, you are writing a hypothesis that suggests that your guess is not true, and that the independent and dependent variables have no relationship .

One null hypothesis for the cell phone and sleep study from the last section might say: 

“If children have more screen usage at bedtime, their quality of sleep and attention will not be worse.” 

In this case, this is a null hypothesis because it’s asking the opposite of the original thesis! 

Conversely, if your if-then hypothesis suggests that your two variables have no relationship, then your null hypothesis would suggest that there is one. So, pretend that there is a study that is asking the question, “Does the amount of followers on Instagram influence how long people spend on the app?” The independent variable is the amount of followers, and the dependent variable is the time spent. But if you, as the researcher, don’t think there is a relationship between the number of followers and time spent, you might write an if-then hypothesis that reads:

“If people have many followers on Instagram, they will not spend more time on the app than people who have less.”

In this case, the if-then suggests there isn’t a relationship between the variables. In that case, one of the null hypothesis examples might say:

“If people have many followers on Instagram, they will spend more time on the app than people who have less.”

You then test both the if-then and the null hypothesis to gauge if there is a relationship between the variables, and if so, how much of a relationship. 

4 Tips to Write the Best Hypothesis

If you’re going to take the time to hold an experiment, whether in school or by yourself, you’re also going to want to take the time to make sure your hypothesis is a good one. The best hypotheses have four major elements in common: plausibility, defined concepts, observability, and general explanation.

#1: Plausibility

At first glance, this quality of a hypothesis might seem obvious. When your hypothesis is plausible, that means it’s possible given what we know about science and general common sense. However, improbable hypotheses are more common than you might think. 

Imagine you’re studying weight gain and television watching habits. If you hypothesize that people who watch more than  twenty hours of television a week will gain two hundred pounds or more over the course of a year, this might be improbable (though it’s potentially possible). Consequently, c ommon sense can tell us the results of the study before the study even begins.

Improbable hypotheses generally go against  science, as well. Take this hypothesis example: 

“If a person smokes one cigarette a day, then they will have lungs just as healthy as the average person’s.” 

This hypothesis is obviously untrue, as studies have shown again and again that cigarettes negatively affect lung health. You must be careful that your hypotheses do not reflect your own personal opinion more than they do scientifically-supported findings. This plausibility points to the necessity of research before the hypothesis is written to make sure that your hypothesis has not already been disproven.

#2: Defined Concepts

The more advanced you are in your studies, the more likely that the terms you’re using in your hypothesis are specific to a limited set of knowledge. One of the hypothesis testing examples might include the readability of printed text in newspapers, where you might use words like “kerning” and “x-height.” Unless your readers have a background in graphic design, it’s likely that they won’t know what you mean by these terms. Thus, it’s important to either write what they mean in the hypothesis itself or in the report before the hypothesis.

Here’s what we mean. Which of the following sentences makes more sense to the common person?

If the kerning is greater than average, more words will be read per minute.

If the space between letters is greater than average, more words will be read per minute.

For people reading your report that are not experts in typography, simply adding a few more words will be helpful in clarifying exactly what the experiment is all about. It’s always a good idea to make your research and findings as accessible as possible. 

Good hypotheses ensure that you can observe the results. 

#3: Observability

In order to measure the truth or falsity of your hypothesis, you must be able to see your variables and the way they interact. For instance, if your hypothesis is that the flight patterns of satellites affect the strength of certain television signals, yet you don’t have a telescope to view the satellites or a television to monitor the signal strength, you cannot properly observe your hypothesis and thus cannot continue your study.

Some variables may seem easy to observe, but if you do not have a system of measurement in place, you cannot observe your hypothesis properly. Here’s an example: if you’re experimenting on the effect of healthy food on overall happiness, but you don’t have a way to monitor and measure what “overall happiness” means, your results will not reflect the truth. Monitoring how often someone smiles for a whole day is not reasonably observable, but having the participants state how happy they feel on a scale of one to ten is more observable. 

In writing your hypothesis, always keep in mind how you'll execute the experiment.

#4: Generalizability 

Perhaps you’d like to study what color your best friend wears the most often by observing and documenting the colors she wears each day of the week. This might be fun information for her and you to know, but beyond you two, there aren’t many people who could benefit from this experiment. When you start an experiment, you should note how generalizable your findings may be if they are confirmed. Generalizability is basically how common a particular phenomenon is to other people’s everyday life.

Let’s say you’re asking a question about the health benefits of eating an apple for one day only, you need to realize that the experiment may be too specific to be helpful. It does not help to explain a phenomenon that many people experience. If you find yourself with too specific of a hypothesis, go back to asking the big question: what is it that you want to know, and what do you think will happen between your two variables?

Hypothesis Testing Examples

We know it can be hard to write a good hypothesis unless you’ve seen some good hypothesis examples. We’ve included four hypothesis examples based on some made-up experiments. Use these as templates or launch pads for coming up with your own hypotheses.

Experiment #1: Students Studying Outside (Writing a Hypothesis)

You are a student at PrepScholar University. When you walk around campus, you notice that, when the temperature is above 60 degrees, more students study in the quad. You want to know when your fellow students are more likely to study outside. With this information, how do you make the best hypothesis possible?

You must remember to make additional observations and do secondary research before writing your hypothesis. In doing so, you notice that no one studies outside when it’s 75 degrees and raining, so this should be included in your experiment. Also, studies done on the topic beforehand suggested that students are more likely to study in temperatures less than 85 degrees. With this in mind, you feel confident that you can identify your variables and write your hypotheses:

If-then: “If the temperature in Fahrenheit is less than 60 degrees, significantly fewer students will study outside.”

Null: “If the temperature in Fahrenheit is less than 60 degrees, the same number of students will study outside as when it is more than 60 degrees.”

These hypotheses are plausible, as the temperatures are reasonably within the bounds of what is possible. The number of people in the quad is also easily observable. It is also not a phenomenon specific to only one person or at one time, but instead can explain a phenomenon for a broader group of people.

To complete this experiment, you pick the month of October to observe the quad. Every day (except on the days where it’s raining)from 3 to 4 PM, when most classes have released for the day, you observe how many people are on the quad. You measure how many people come  and how many leave. You also write down the temperature on the hour. 

After writing down all of your observations and putting them on a graph, you find that the most students study on the quad when it is 70 degrees outside, and that the number of students drops a lot once the temperature reaches 60 degrees or below. In this case, your research report would state that you accept or “failed to reject” your first hypothesis with your findings.

Experiment #2: The Cupcake Store (Forming a Simple Experiment)

Let’s say that you work at a bakery. You specialize in cupcakes, and you make only two colors of frosting: yellow and purple. You want to know what kind of customers are more likely to buy what kind of cupcake, so you set up an experiment. Your independent variable is the customer’s gender, and the dependent variable is the color of the frosting. What is an example of a hypothesis that might answer the question of this study?

Here’s what your hypotheses might look like: 

If-then: “If customers’ gender is female, then they will buy more yellow cupcakes than purple cupcakes.”

Null: “If customers’ gender is female, then they will be just as likely to buy purple cupcakes as yellow cupcakes.”

This is a pretty simple experiment! It passes the test of plausibility (there could easily be a difference), defined concepts (there’s nothing complicated about cupcakes!), observability (both color and gender can be easily observed), and general explanation ( this would potentially help you make better business decisions ).

Experiment #3: Backyard Bird Feeders (Integrating Multiple Variables and Rejecting the If-Then Hypothesis)

While watching your backyard bird feeder, you realized that different birds come on the days when you change the types of seeds. You decide that you want to see more cardinals in your backyard, so you decide to see what type of food they like the best and set up an experiment. 

However, one morning, you notice that, while some cardinals are present, blue jays are eating out of your backyard feeder filled with millet. You decide that, of all of the other birds, you would like to see the blue jays the least. This means you'll have more than one variable in your hypothesis. Your new hypotheses might look like this: 

If-then: “If sunflower seeds are placed in the bird feeders, then more cardinals will come than blue jays. If millet is placed in the bird feeders, then more blue jays will come than cardinals.”

Null: “If either sunflower seeds or millet are placed in the bird, equal numbers of cardinals and blue jays will come.”

Through simple observation, you actually find that cardinals come as often as blue jays when sunflower seeds or millet is in the bird feeder. In this case, you would reject your “if-then” hypothesis and “fail to reject” your null hypothesis . You cannot accept your first hypothesis, because it’s clearly not true. Instead you found that there was actually no relation between your different variables. Consequently, you would need to run more experiments with different variables to see if the new variables impact the results.

Experiment #4: In-Class Survey (Including an Alternative Hypothesis)

You’re about to give a speech in one of your classes about the importance of paying attention. You want to take this opportunity to test a hypothesis you’ve had for a while: 

If-then: If students sit in the first two rows of the classroom, then they will listen better than students who do not.

Null: If students sit in the first two rows of the classroom, then they will not listen better or worse than students who do not.

You give your speech and then ask your teacher if you can hand out a short survey to the class. On the survey, you’ve included questions about some of the topics you talked about. When you get back the results, you’re surprised to see that not only do the students in the first two rows not pay better attention, but they also scored worse than students in other parts of the classroom! Here, both your if-then and your null hypotheses are not representative of your findings. What do you do?

This is when you reject both your if-then and null hypotheses and instead create an alternative hypothesis . This type of hypothesis is used in the rare circumstance that neither of your hypotheses is able to capture your findings . Now you can use what you’ve learned to draft new hypotheses and test again! 

Key Takeaways: Hypothesis Writing

The more comfortable you become with writing hypotheses, the better they will become. The structure of hypotheses is flexible and may need to be changed depending on what topic you are studying. The most important thing to remember is the purpose of your hypothesis and the difference between the if-then and the null . From there, in forming your hypothesis, you should constantly be asking questions, making observations, doing secondary research, and considering your variables. After you have written your hypothesis, be sure to edit it so that it is plausible, clearly defined, observable, and helpful in explaining a general phenomenon.

Writing a hypothesis is something that everyone, from elementary school children competing in a science fair to professional scientists in a lab, needs to know how to do. Hypotheses are vital in experiments and in properly executing the scientific method . When done correctly, hypotheses will set up your studies for success and help you to understand the world a little better, one experiment at a time.

What’s Next?

If you’re studying for the science portion of the ACT, there’s definitely a lot you need to know. We’ve got the tools to help, though! Start by checking out our ultimate study guide for the ACT Science subject test. Once you read through that, be sure to download our recommended ACT Science practice tests , since they’re one of the most foolproof ways to improve your score. (And don’t forget to check out our expert guide book , too.)

If you love science and want to major in a scientific field, you should start preparing in high school . Here are the science classes you should take to set yourself up for success.

If you’re trying to think of science experiments you can do for class (or for a science fair!), here’s a list of 37 awesome science experiments you can do at home

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Ashley Sufflé Robinson has a Ph.D. in 19th Century English Literature. As a content writer for PrepScholar, Ashley is passionate about giving college-bound students the in-depth information they need to get into the school of their dreams.

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Biology archive

Course: biology archive   >   unit 1, the scientific method.

• Controlled experiments
• The scientific method and experimental design

Introduction

• Make an observation.
• Ask a question.
• Form a hypothesis , or testable explanation.
• Make a prediction based on the hypothesis.
• Test the prediction.
• Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation., 2. ask a question., 3. propose a hypothesis., 4. make predictions., 5. test the predictions..

• If the toaster does toast, then the hypothesis is supported—likely correct.
• If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

• If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
• If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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Definition of a Hypothesis

What it is and how it's used in sociology

• Key Concepts
• Major Sociologists
• News & Issues
• Research, Samples, and Statistics
• Recommended Reading
• Archaeology

A hypothesis is a prediction of what will be found at the outcome of a research project and is typically focused on the relationship between two different variables studied in the research. It is usually based on both theoretical expectations about how things work and already existing scientific evidence.

Within social science, a hypothesis can take two forms. It can predict that there is no relationship between two variables, in which case it is a null hypothesis . Or, it can predict the existence of a relationship between variables, which is known as an alternative hypothesis.

In either case, the variable that is thought to either affect or not affect the outcome is known as the independent variable, and the variable that is thought to either be affected or not is the dependent variable.

Researchers seek to determine whether or not their hypothesis, or hypotheses if they have more than one, will prove true. Sometimes they do, and sometimes they do not. Either way, the research is considered successful if one can conclude whether or not a hypothesis is true. 

Null Hypothesis

A researcher has a null hypothesis when she or he believes, based on theory and existing scientific evidence, that there will not be a relationship between two variables. For example, when examining what factors influence a person's highest level of education within the U.S., a researcher might expect that place of birth, number of siblings, and religion would not have an impact on the level of education. This would mean the researcher has stated three null hypotheses.

Alternative Hypothesis

Taking the same example, a researcher might expect that the economic class and educational attainment of one's parents, and the race of the person in question are likely to have an effect on one's educational attainment. Existing evidence and social theories that recognize the connections between wealth and cultural resources , and how race affects access to rights and resources in the U.S. , would suggest that both economic class and educational attainment of the one's parents would have a positive effect on educational attainment. In this case, economic class and educational attainment of one's parents are independent variables, and one's educational attainment is the dependent variable—it is hypothesized to be dependent on the other two.

Conversely, an informed researcher would expect that being a race other than white in the U.S. is likely to have a negative impact on a person's educational attainment. This would be characterized as a negative relationship, wherein being a person of color has a negative effect on one's educational attainment. In reality, this hypothesis proves true, with the exception of Asian Americans , who go to college at a higher rate than whites do. However, Blacks and Hispanics and Latinos are far less likely than whites and Asian Americans to go to college.

Formulating a Hypothesis

Formulating a hypothesis can take place at the very beginning of a research project , or after a bit of research has already been done. Sometimes a researcher knows right from the start which variables she is interested in studying, and she may already have a hunch about their relationships. Other times, a researcher may have an interest in ​a particular topic, trend, or phenomenon, but he may not know enough about it to identify variables or formulate a hypothesis.

Whenever a hypothesis is formulated, the most important thing is to be precise about what one's variables are, what the nature of the relationship between them might be, and how one can go about conducting a study of them.

Updated by Nicki Lisa Cole, Ph.D

• Null Hypothesis Examples
• Examples of Independent and Dependent Variables
• Difference Between Independent and Dependent Variables
• The Difference Between Control Group and Experimental Group
• What Is a Hypothesis? (Science)
• Understanding Path Analysis
• What Are the Elements of a Good Hypothesis?
• What It Means When a Variable Is Spurious
• What 'Fail to Reject' Means in a Hypothesis Test
• How Intervening Variables Work in Sociology
• Null Hypothesis Definition and Examples
• Understanding Simple vs Controlled Experiments
• Scientific Method Vocabulary Terms
• Null Hypothesis and Alternative Hypothesis
• Six Steps of the Scientific Method
• What Are Examples of a Hypothesis?

Cleaning the bilge tanks: Is Brazil on the route of international oceanic dumping?

• Zacharias, Daniel
• Crespo, Natália
• da Silva, Natália
• da Rocha, Rosmeri
• Gama, Carine
• Ribeiro e Silva, Sérgio
• Harari, Joseph

In 2019-2020, a "Mysterious" oil spill reached almost 3,000 km of the Brazilian shore. Despite the large affected area, the STFM (Spill, Transport and Fate Model) [1] time-reverse modeling indicated a relatively small Venezuelan oil volume (5000-12,500 m3) [2] and quite far from the coast [3]. These volume and position are consistent with a cleaning bilge tank procedure.In the following years, at least three similar events were recorded, one on the equatorial coast of Brazil and two in the Fernando de Noronha archipelago (about 375 km from the coast). These events indicated that Brazil was being periodically reached by tar balls and oil slicks from unknown origin. The most likely routes were mapped and computationally tested [3] [4].The hypothesis is that the oil/waste dumped in international waters by ships on-route to Cape of Good Hope is reaching the Brazilian coast. On that account, 9,000 probabilistic simulations (distributed in 30-year of data), each one with 20,000 Lagrangian elements, were used to estimate the probability of dumped oil residue reaching the Brazilian coast. About 20,000 - 35,000 ships navigate this route and the modeling results have shown that up to 28.5 % of large ships could dump oil on-route towards Cape of Good Hope. Inside the Brazilian Exclusive Economic Zone, the probability of dumped oil/waste reaching the coastline is about 62 % and quickly decreases for more distant dumping zones (Zones 2 and 3). Equatorial and Northeast shores of Brazil are the most vulnerable to oceanic dumping when compared to other regions.Brazilian Federal Police declared that a Greek-flagged tanker (i.e., Bouboulina) is the main suspect of the 2019's oil spill [5]. However, the simulation results suggest an alternative hypothesis: The City of Tokyo (VL Nichioh) tanker that crossed Zone 2 area between June 18th and 20th, 2019, towards Venezuela to be loaded. The drift time (72 days) is compatible with the position, also, the loading that would take place in a few days could motivate the tanker to execute a cleaning procedure, accumulating a large volume of residual oil in the bilge tank [4]. [1] Zacharias, D.C., et al., 2018. Offshore petroleum pollution compared numerically via algorithm tests and computation solutions. Ocean Eng., https://doi.org/10.1016/j.oceaneng.2018.01.007. [2] Zacharias, D.C., et al., 2021a. Mysterious oil spill on Brazilian coast: analysis and estimates. Mar. Pollut. Bull., https://doi.org/10.1016/j.marpolbul.2021.112125. [3] Zacharias, D.C., et al., 2021b. Mysterious oil spill on the Brazilian coast - part 2: a probabilistic approach to fill gaps of uncertainties. Mar. Pollut. Bull., https://doi.org/10.1016/j.marpolbul.2021.113085.[4] Zacharias, D.C., et al., 2023. Oil reaching the coast: Is Brazil on the route of international oceanic dumping? Mar. Pollut. Bull., https://doi.org/10.1016/j.marpolbul.2023.115624. [5] Escobar, H., (2019), Mysterious oil spill threatens marine biodiversity haven in Brazil, Science, https://www.science.org/doi/full/10.1126/science.366.6466.672. Keywords: STFM; Oil spill; Oil dumping

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