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PhD candidates pursuing their doctorates at Radboud University have the opportunity to publish their dissertations with Radboud University Press as part of the Radboud Dissertation Series. The individual dissertations will gain visibility within their specific field of study through indexing.

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Date: June 20 2024 Time: 10:00-11:00 AM and 12:30-13:30 PM Location: UB, Erasmuslaan 36, UBN.1.05b, Nijmegen

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Analytical Methods

New insight from maldi-tof ms and multivariate data analysis on the botanical origin of polysaccharide-based paint binders in ancient egypt †.

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* Corresponding authors

a Art Institute of Chicago, Department of Conservation and Science, 111 S. Michigan Ave, Chicago, IL 60603, USA E-mail: [email protected]

b Center for Scientific Studies in the Arts, Northwestern University, Tech Building, 2145 Sheridan Road, Evanston, IL 60208, USA

c Radboud University, Institute for Molecules and Materials, (Analytical Chemistry & Chemometrics), P. O. Box 9010, Nijmegen, 6500 GL, The Netherlands

Polysaccharide-based materials of plant origin are known to have been used as binding media in paint and ground layers of artifacts from ancient Egypt, including wall paintings, cartonnages and sarcophagi. The use of gums from Acacia , Astragalus and Prunus genera has been suggested in the literature on the basis of their qualitative or quantitative monosaccharide profile after complete chemical hydrolysis. The introduction of partial enzymatic digestion of the polysaccharide material, followed by analysis of the released oligosaccharides by matrix assisted laser desorption ionization-time-of-flight mass spectrometry, has proved effective in discriminating among gums from different genera, as well as among species within the Acacia genus. In this study, the previously built Acacia database was expanded, principal component analysis (PCA) was used to aid in grouping of the samples, and data interpretation was refined following a modified acacieae taxonomy. Application of the analytical strategy to investigate the paint binders in artworks from ancient Egypt allowed qualitative discrimination of gums at a species level, and provided new insights into the artists' material choices.

Graphical abstract: New insight from MALDI-TOF MS and multivariate data analysis on the botanical origin of polysaccharide-based paint binders in ancient Egypt

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thesis radboud

New insight from MALDI-TOF MS and multivariate data analysis on the botanical origin of polysaccharide-based paint binders in ancient Egypt

C. Granzotto, A. Aksamija, G. H. Tinnevelt, V. Turkina and K. Sutherland, Anal. Methods , 2024, Advance Article , DOI: 10.1039/D3AY02214E

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